Research Specialist in Chemistry
Roy J. Carver Biotechnology Center University of Illinois- Urbana-Champaign
The Specialist will conduct protein analysis with and on behalf of Director of Proteomics
The required experience and qualifications are: M.S. in Chemistry/Biochemistry. Alternative degree fields will be considered/accepted if accompanied by equivalent experience (depending on nature and depth of experience as it relates to the position). Must be able to perform the following: protein fractionation, purification, chromatography, characterization (e.g. electrophoresis, HPLC), chemical modifications, hydrolysis (acid/alkali), enzyme digestions (e.g. traditional and microwave) and mass spectrometry. Mass spectrometry analysis include MALDI ToF, ESI LC/MS, and direct infusion. Techniques required are protein preparation, sample cleanup (e.g. SPE, affinity column etc.), in-gel protein digestions, protein solution digestions, HPLC (e.g. preparative, analytical, capillary and nano), stable isotope tag labeling, familiarity with Orbitrap class mass spectrometers, familiarity and experience using Thermo Scientific software (Xcalibur, Proteome Explorer, Matrix Sciences Mascot Distiller and Server, Scaffold analysis and reporting software. Must be organized, possess excellent communication skills. Work both as a team and independently.
The preferred: Ph.D. degree. Five or more years of analytical chemistry and hands-on Proteomics experience. Additional experiences with techniques such as iTRAQ, SILAC, TMT, MaxQuant Analysis, Biacore (SPR) and proteomics LIMS are preferred.
Major duties and responsibilities:
-Provide sample cleanup (SPE and affinity column), in-gel protein digestion, protein solution digestions, hydrolysis (acid/alkali), enzyme digestions (traditional and microwave), lyophilization, HPLC (preparative, analytical, capillary, and nano), stable isotope tag labeling.
- Preparation of proteins and peptides by Fractionation, separation, measurement, and identification using electrophoresis, HPLC, FPLC and mass spectrometry.
- Perform mass spectrometric analysis using Fusion™ Orbitrap MS, MALDI ToF MS, ESI, LC/MS, Advion Triversa Nanomate robot infusion.
- Responsible for designing, performing, analysis, and training users for Surface Plasmon Resonance (Biacore) experiment.
- Responsible for providing, interpretation, and analysis of experimental results.
- Keep abreast of new equipment, materials, methodologies and techniques in protein and peptide chemistry that are relevant to the services of the facility.
- Maintain laboratory supply stock (e.g. chemicals, tube/glassware, etc.)
- Responsible for providing data to Facility Director for implementation of billing and pricing system, and serve as back-up for processing these systems.
Type of Position: Full-time Academic Professional with full University benefits package. Salary is commensurate with experience.
Application: Please complete your profile at http://jobs.illinois.edu and upload your cover letter and resume, to include at least three contacts for reference. All requested information must be submitted for your application to be considered.
For additional information, contact Dr. Peter Yau, Director of Proteomics.
Phone: (217)333-3841; firstname.lastname@example.org
For full consideration, applications must be received by Friday, June 9th.
The University of Illinois conducts criminal background checks on all job candidates upon acceptance of a contingent offer.
Illinois is an equal opportunity employer and all qualified applicants will receive consideration for employment without regard to race, religion, color, national origin, sex, sexual orientation, gender identity, age, status as a protected veteran, status as a qualified individual with a disability, or criminal conviction history. Illinois welcomes individuals with diverse backgrounds, experiences, and ideas who embrace and value diversity and inclusivity. (www.inclusiveillinois.illinois.edu).
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