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Protein Sciences

Peter Yau, Ph.D - Director

315 Noyes Laboratory,

505 South Mathews Avenue, Urbana, IL 61801

Phone: (217) 333-3841 

Email: pmyau@illinois.edu

Protein Sciences Facility Pricing

Prior to project initiation, a meeting is required with CBC staff to discuss each project. If mutually agreed that the CBC can support the project, we will deliver a quote outlining the approach, timeline, and cost for the agreed service(s). As this is cutting-edge science, we cannot guarantee success, in all cases the user will be billed for cost and labor for CBC effort per our fee structure. After acceptance of the quote, the user will fill out and return the sample submission form provided with the quote, and also found on each CBC facility’s website. All samples are assumed to be safe, and no greater than BSL1, unless otherwise agreed upon in advance. The user must diligently and transparently followed all University of Illinois sample acquisition, movement, and safety protocols.

 

Prices effective July 1, 2022

 

Preparative Liquid Chromatography

 

FPLC

     Buffer Preparation

     Fraction Collection

$194.00/injection

$25.00-50.00/analysis

$22.00/plate

Self Service

 

Typhoon 9400 Imaging (half hour minimum)

$89.00/half hour

Intact Mass Analysis with Advion TriVersa and Fusion
(2 hour minimum, prior training required)

$132.00/hour + $5.00/nozzle

Intact Mass Analysis with Advion TriVersa and UHMR
(2 hour minimum, prior training required)

$142.00/hour + $5.00/nozzle

Enzymatic Digestion

 

Trypsin (default unless otherwise noted)

$53.00/sample

Glu-C

$100.00/sample

Lys-C

$53.00/sample
LysC + Trypsin sequential digest

$100.00/sample

Other proteases (chymotrypsin, thermolysin, etc.)

$100.00/sample

Additional Proteomic Sample Preparation

 

Protein Extraction

$62.00/sample
Reduction & Alkylation

$41.00/sample

Sample Desalting (StageTip)

$38.00/sample

Detergent Removal

$50.00/sample

Protein Precipitation

$50.00/sample

C18 Column (Sep-Pak)

$60.00/sample

Buffer Exchange (Spin column)

$38.00/sample

Phosphorylation Enrichment (Fe-IMAC or TiO2)

$74.00/sample

Protein Concentration Measurement (BCA Assay)

$65.00/plate

Mass Spectrometry

 

LC-MS/MS (45 min rapid analysis)

$124.00/sample

LC-MS/MS (60 min standard analysis)

$165.00/sample

LC-MS/MS (90 min analysis)

$186.00/sample

LC-MS/MS (120 min extended analysis)

$207.00/sample

LC-MS/MS (180 min analysis)

$248.00/sample
In-gel bundle for on-campus projects (CEM digest + rapid LC-MS/MS)

$158.00/sample

Quantitative Proteomic Analysis

    Label-free

    Isotopic label (iTRAQ, TMT, dimethyl labeling, etc.)

inquire for quote

Intact protein analysis*

$132.00/sample

Lyophilization 

 

5 mL and under

$54.00/sample

5 mL to 200 mL

$60.00/sample

Additional Data Analysis

$52.00/hour

Custom Database or Modification Added to Mascot Server

$13.00/addition

*Prior consultation recommended

External users are charged an additional 31.7% overhead fee, which is capped at 20% for those users from State of Illinois subsidized universities and government agencies. Off-campus projects require an off-campus user testing agreement, to be provided by the facility, and if over $10,000 a technical testing agreement. For further information contact Dr. Mark A. Mikel (217-244-0144; mmikel@illinois.edu ), Associate Director of the Roy J. Carver Biotechnology Center.

All scientific work performed by the Roy J. Carver Biotechnology Center (CBC) is required to be acknowledged in scholarly publications, posters, and presentations.  Proper recognition allows us to measure the impact of our work and supports our initiatives in obtaining sponsored funding. In addition, any CBC personnel who make a substantial intellectual or experimental contribution are deserving of further recognition as co-author.