Service/Equipment
Real Time PCR QuantStudio 7
The QuantStudio 7 real time PCR machine is operational and available for use by all university researchers. The use of low volume reactions and 384 well plates conserves both reagents and budget. Taqman Low Density Arrays (TLDA) can also be run on the QuantStudio. In addition, our machine is equipped with an autoloading robot allowing 24 hour use of the machine. Real time PCR is one of the most sensitive and accurate assays for RNA expression analysis, allele discrimination or DNA quantification. At present, all users are trained for sample setup and analysis by Functional Genomics Lab staff. Users prepare their samples independently and bring in their PCR plates ready to run. Sequence Analysis software can be downloaded from the Thermo Fisher website. Please contact Tatsiana Akraiko (akraiko@illinois.edu) to set up a training session.
For University of Illinois researchers there is a $45.00 fee/plate. This covers only maintenance and service contracts. Please provide an account number when making an appointment to use the machine. Plasticware and reagents required for Real-Time PCR analysis
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QuantStudio Consumables List |
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| ABI 384 well plates: | 384-Well Clear Optical Reaction Plate (with Barcode, code 128) Part Number 4309849 50 plates/box |
| Optical Adhesive Cover Starter Kit | Part Number 4313663: $39.75 20 adhesive covers 1 compression pad 1 Cover Applicator |
| ABI PRISM Optical Adhesive Covers | Part Number: 4311971 100 Covers/box |
| ABI SYBER Green Mastermix | Part Number: 4309155 5 ml. |
| USB Flash Drive | Flash drive is probably the most convenient method for transferring data with the 7900HT. The machine is not connected to the network for security reasons. |
We strongly suggest reading the following technical bulletins published by Applied Biosystems explaining the principles of Real-Time PCR before planning your assays.
- **New** Real Time PCR User Instructions
- Designing MGB Probes and Primers
- Essentials of realtime PCR
- Guide to relative quantitation
- Rapid Assay developement guidelines
- Real time vs. traditional PCR
- User bulletin 2
For more information contact Dr. Mark Band at the Functional Genomics Lab. Tel: (217)244-3930
E-mail: markband@illinois.edu
All work performed by the Roy J. Carver Biotechnology Center (CBC) should be acknowledged in scholarly publications, posters, and presentations. Proper recognition allows us to measure the impact of our work and supports our initiatives in obtaining sponsored funding. In addition, any CBC personnel who make a substantial intellectual or experimental contribution are deserving of further recognition as co-author.