10x Xenium In situ System
Xenium is an end-to-end platform from 10x Genomics that provides highly sensitive, targeted gene and protein expression information at subcellular resolution. The Xenium Analyzer combines the power of single molecule RNA and protein detection with powerful optics, data acquisition, and decoding technology to rapidly detect analytes at high plexy and at subcellular resolution across entire tissue sections. The platform is designed to be compatible with both fresh frozen (FF) and formalin-fixed, paraffin-embedded (FFPE) tissues
Xenium In Situ workflow overview
Xenium In Situ: High-performance in situ from the single cell leader
Xenium Sample information | |
Imageable Sample area | Total sample area inside the fiducials is 10.45mm x 22.45mm. |
Tissue compatibility | Fresh Frozen, FFPE, and Fixed frozen |
Sample type | Mouse and human brain, human lung, human breast, human skin, human colon, as well as multi-tissue panels for both human and mouse samples are available. Human Immuno-Oncology Profiling Panel is now available |
Restricted thickness | Fresh Frozen = 10 µM; FFPE = 5 µM |
Data analysis | Xenium explorer, Xenium ranger, third party tools |
Downstream processing | Enables tissue morphology (H/E) staining, or Visium spatial transcriptome analysis and immunofluorescence (IF), on the same tissue section. |
Xenium supported panels:
Post-Xenium In Situ Applications: Immunofluorescence, H&E, and Visium CytAssist Spatial Gene Expression
For more information about the Xenium platform:
Gene expression panels on xenium
Xenium software updates and important notes
FFPE Human breast tissue on Xenium
Manufacturer: 10X Genomics
Equipment Model: 10x Xenium Platform
Location: Main Facility at 231 Edward R. Madigan Laboratory
Contact Person: Mayandi Sivaguru, sivaguru@illinois.edu
