About Us
Do you Know Everything that the DNA Sequencing Services Laboratory can do for you?
Located within the Roy J. Carver Biotechnology Center, the DNA Services Laboratory is equipped with state-of-the-art Next Generation Sequencing technologies, including Illumina NovaSeq X Plus and several MiSeq, PacBio Revio, 10x Genomics Single-Cell, and Oxford Nanopore.
Illumina NovaSeq X Plus and 6000
The NovaSeq X Plus is the latest instrument from Illumina for ultra-high throughput sequencing of genomic DNA, RNA-Seq, DNA methylation, targeted sequencing, and many other samples that need high sequencing depth at the lowest possible cost.
The NovaSeq 6000 performs 2x250nt runs, which are still not available in the NovaSeq X Plus.
PacBio REVIO
This is the latest instrument from PacBio, it produces the longest reads with the highest accuracy of all long-reads. It is the instrument of choice for assembly of complex genomes, metagenomes, genome resequencing with long reads, full-length 16S and other amplicons and full-length transcriptomes (IsoSeq).
One REVIO Cell produces
- up to 120 Gbases of HiFi error-corrected reads with fragments 10-30kb
- up to 13 million error-corrected single long reads.
- up to 100 million concatenated 16S, full-length cDNA, or amplicon reads.
Illumina MiSeq i100 and MiSeq
For sequencing libraries from amplicons (ie: 16S, 18S, ITS, other), genomic DNA, microbial DNA, etc.
- The new MiSeq i100 has two kits, the 5M (5 million read-pairs) and the 25M (25 million read-pairs)
- The read-lengths of the MiSeq i100 are up to 300bp from each side of the DNA fragments
- MiSeq V2 Nano run produces 500k to 2 million paired-reads
- MiSeq V2 bulk run produces 8 to 20 million paired-reads
- MiSeq V3 run produces 10 to 40 million paired-reads
Oxford Nanopore GridION
Long sequencing reads from genomic DNA, RNA, cDNA, 16S or other amplicons.
- One flowcell produces from 1 to 9 million reads depending on the type of sample.
- Reads are typically from 5kb to 40kb and with some reads from HMW genomic DNA reaching up to 100kb.
- Each flowcell runs one or multiple samples.
10x Genomics
The 10x Chromium X system produces libraries for 5′ or 3′ transcriptome sequencing from single-cells, ATAC-seq, and multiomics (RNAseq + ATACseq). Fixed probe-based libraries compatible with human/mouse samples are also available.
- Libraries constructed on 10x are fully compatible with Illumina sequencing.
- Single-cell libraries can be constructed from 500 to 20,000 cells per library.
- Single-cell libraries are sequenced for 28x90nt or 28x150nt on the NovaSeq, with a minimum of 50k reads per cell recommended.
- 10x Visium Spatial gene expression libraries are also available.
- 10x Xenium in situ platform is also available in our sister Core, CMtO.
Fragment Analyzers and Femto Pulse
Qualitative analysis of gDNA, RNA, amplicons, or finished libraries using capillary electrophoresis.
- RQN (RNA Quality Scores)
- 12-, 48-, and 96-capillary instruments for best pricing depending on sample size/need.
- QC can be performed typically with 24 hours.
We offer advanced capabilities for sample and library preparation across a wide range of genomics
applications, including:
- Differential gene expression (RNA-seq).
- Genome resequencing for variant characterization.
- Extraction and Sequencing of cell-free DNA and cell-free miRNA.
- Single-cell RNA-seq, ATAC-seq, multi-omics, and whole genome methylation.
- De novo genome assembly.
- Epigenomics applications such as whole genome methylation, ATAC-seq, Cut&Run.,
methylation arrays. - Characterization of metagenomic communities with 16S and many other amplicons, and with
whole genome sequencing, and metatranscriptomics. - High-throughput DNA and RNA extractions.
- Sanger capillary sequencing.
- Many custom applications. Ask!
These services are available to all researchers, including faculty, postdoctoral fellows, graduate students, and undergraduates. And yes, we accept off-campus projects as well, which helps to keep costs lower for everyone.
Sequencing prices and options evolve frequently. Please contact us for current quotations. Prices have decreased significantly with the acquisition of new instruments. Even if you have just one or two samples, we have options to make it work within our facility. There is no need to go through the hassle of generating a PO and Purchasing! All we need is the on-campus CFOP. Off-campus users (academic, private, federal) work with us through a simple contract.
In addition to sequencing services, our staff provides:
- Project quotes tailored to various scenarios and sample numbers.
- Departmental seminars.
- Letters of support and materials and methods sections for grant applications.
- Materials and methods sections for publications based on work conducted in our facility.
- Individual tours for faculty and lab members (ask for these!).
- Participation in lectures on sequencing technologies.
NovaSeq X Plus and 6000 Sequencing Options (see full list with multi-lane discounts in Pricing link above)
| Flowcell | Read Type and Length | Price Per Lane | # of Single-Reads or Read Pairs Per lane | Total # of Reads | Gb/lane |
|---|---|---|---|---|---|
| X Plus 10B | Single-reads, 100bp | $1,690 | 1 billion | 1 billion | 150+ |
| X Plus 10B | Paired-reads, 2x150nt | $2,190 | 1 billion | 2 billion | 300+ |
| X Plus 25B | Paired-reads, 2x150nt | $3,290 | 3.2 billion | 6.4 billion | 900+ |
| 6000 SP | Paired-reads, 2x250nt | $3,440 | 400 million | 800 million | 200+ |
We are available for consultations regarding experimental design, sample submission, cost estimates, and other aspects to help ensure high-quality results and efficient turnaround. We also welcome suggestions for additional services that may better support your research needs.
Please feel free to contact us to discuss how we can support your research:
Alvaro G. Hernandez, Ph.D.
Director of DNA Services
aghernan@illinois.edu
(217) 244-3480
Chris L. Wright
Associate Director of DNA Services
clwright@illinois.edu
(217) 333-4372
Visit our website: https://biotech.illinois.edu/dna-services-core/