DNA Pricing
Pricing
Prior to project initiation, a meeting is required with CBC staff to discuss each project. If mutually agreed that the CBC can support the project, we will deliver a quote outlining the approach, timeline, and cost for the agreed service(s). As this is cutting-edge science, we cannot guarantee success. In all cases the user will be billed for cost and labor for CBC effort per our fee structure. After acceptance of the quote, the user will fill out and return the sample submission form provided with the quote. All samples are assumed to be safe, and no greater than BSL1, unless otherwise agreed upon in advance. The user must diligently and transparently follow all University of Illinois sample acquisition, movement, and safety protocols.
All pricing is updated, effective July 1st, 2025. Listed rates do not include the off-campus overhead (see bottom of page for overhead details).
Illumina NovaSeq X Plus and 6000: Ultra high-throughput sequencing of mRNA, genomic DNA, ChIP-DNA, small RNAs, bisulfite-treated DNA, single-cell transcriptomics, etc. For descriptions of library construction requirements and sequencing platform metrics, please visit our Sample Submission Page.
PacBio REVIO: accurate long reads for de novo genome assembly and sequencing of long amplicons or cDNA.
EXTRACTION & QC
| Service | Price |
| Hourly rate, cell test counts, other (per hour) | $119 |
| Quality Check (QC) – one library (Qubit, Fragment Analyzer & qPCR) | $114 |
| Quality Check (QC) – 2-8 libraries (Qubit, Fragment Analyzer & qPCR) | $264 |
| Library Purification (eGel or SPRI bead. Does not include QC) | $152 |
| Sample QC: Qubit & Fragment Analyzer (Minimum charge is for 8 samples) | $14 |
| Fragment Analyzer service (up to 96 samples or libraries) | $434 |
| Fragment Analyzer service (up to 48 samples or libraries) | $299 |
| Fragment Analyzer service (1-12 samples or libraries) | $107 |
| FemtoPulse (1-11 samples or libraries) | $159 |
| HMW DNA Size Selection (1-2) | $256 |
| HMW DNA Extraction (40kb+, 1 sample) | $685 |
| HMW DNA Extraction (40kb+, 2+ samples,each) | $440 |
| RNA/DNA Extraction (each, 1-23) | $69 |
| RNA/DNA Extraction (each, 24-47) | $61 |
| RNA/DNA Extraction (each, 48-95) | $34 |
| RNA/DNA Extraction (each, 96-191) | $28 |
| RNA/DNA Extraction (each, 192+) | $22 |
| Covaris Annual User fee | $269 |
| Service | Price |
|---|---|
|
Hourly rate, cell test counts, other (per hour) |
$119 |
|
Quality Check (QC) – one library (Qubit, Fragment Analyzer & qPCR) |
$114 |
|
Quality Check (QC) – 2-8 libraries (Qubit, Fragment Analyzer & qPCR) |
$264 |
|
Library Purification (eGel or SPRI bead. Does not include QC) |
$152 |
|
Sample QC: Qubit & Fragment Analyzer (Minimum charge is for 8 samples) |
$14 |
|
Fragment Analyzer service (up to 96 samples or libraries) |
$434 |
|
Fragment Analyzer service (up to 48 samples or libraries) |
$299 |
|
Fragment Analyzer service (1-12 samples or libraries) |
$107 |
|
FemtoPulse (1-11 samples or libraries) |
$159 |
|
HMW DNA Size Selection (1-2) |
$256 |
|
HMW DNA Extraction (40kb+, 1 sample) |
$685 |
|
HMW DNA Extraction (40kb+, 2+ samples,each) |
$440 |
|
RNA/DNA Extraction (each, 1-23) |
$69 |
|
RNA/DNA Extraction (each, 24-47) |
$61 |
|
RNA/DNA Extraction (each, 48-95) |
$34 |
|
RNA/DNA Extraction (each, 96-191) |
$28 |
|
RNA/DNA Extraction (each, 192+) |
$22 |
|
Covaris Annual User fee |
$269 |
SHOTGUN LIBRARIES
| Service | Price |
| BULK ATAC-Seq ActiveMotif library, <24 (test counts charged separately) | $171 |
| BULK ATAC-Seq ActiveMotif library, >24 (test counts charged separately) | $111 |
| HiC Titration (per sample) | $188 |
| HiC library prep, each | $718 |
| 3D Genomics library prep | $336 |
| Shotgun genomic PCR-free prep, each | $167 |
| Shotgun genomic DNA library prep, each (1-11) | $136 |
| Shotgun genomic DNA library prep, each (12-23) | $102 |
| Shotgun genomic DNA library prep, each (24-47) | $86 |
| Shotgun genomic DNA library prep, each (48-95) | $74 |
| Shotgun genomic DNA library prep, each (96+) | $55 |
| SkimSeq Libraries, each (48+) | $22 |
| SkimSeq Libraries, each (48+; plus QC) | $26 |
RNA-SEQ LIBRARIES
| Service | Price |
| RNA-Seq library, PolyA selected RNA, each | $108 |
| RNA-Seq library, PolyA selected RNA (>12 on robot, full QC) | $86 |
| RNA-Seq library, PolyA selected RNA (>24 on robot, full QC) | $76 |
| RNA-Seq library, PolyA selected RNA (>48 on robot, full QC) | $70 |
| RNA-Seq library, PolyA selected RNA (>96 on robot, full QC) | $64 |
| RNA-Seq library, PolyA selected RNA (>96 on robot, no QC) | $53 |
| RNA-Seq library, rRNA depleted, each | $148 |
| RNA-Seq library, rRNA depleted (>12 on robot, full QC) | $126 |
| RNA-Seq library, rRNA depleted (>24 on robot, full QC) | $100 |
| RNA-Seq library, rRNA depleted (>48 on robot, full QC) | $95 |
| RNA-Seq library, rRNA depleted (>96 on robot, full QC) | $89 |
| RNA-Seq rRNA depleted, mixed probes/Globin/Epidem. add-on, each | $20 |
| Small RNA libraries, each | $181 |
| Small RNA libraries (>24 on robot) | $136 |
| Small RNA libraries (>48 on robot) | $125 |
| Small RNA libraries (>96 on robot) | $113 |
| Bacterial Small RNA libraries (>24 on robot) | $182 |
| Bacterial Small RNA libraries (>48 on robot) | $159 |
| Bacterial Small RNA libraries (>96 on robot) | $136 |
BISULFITE, EXOME, EM-SEQ
| Service | Price |
| Exome Capture libraries (does not inc. probes) | $194 |
| Exome Capture libraries (>48 on robot) | $134 |
| Exome Capture Probes | variable |
| Bisulfite, reduced representation DNA libraries, each | Ask for pricing |
| Bisulfite, reduced representation DNA libraries (>24 on robot) | Ask for pricing |
| Bisulfite, reduced representation DNA libraries (>48 on robot) | Ask for pricing |
| EM-Seq (1-23) | $177 |
| EM-Seq (24+) | $106 |
10X, TELL-SEQ
| Service | Price |
| TELLSeq library construction, 1 library | $957 |
| TELLSeq library construction, 2 libraries | $590 |
| TELLSeq library construction,3-4 libraries | $297 |
| 10X Single Cell Library Prep (any; kit provided) | $441 |
| 10x Feature Barcoding add-on (per sample) | $272 |
| 10X Single Cell RNA Library (1 library) | $2,570 |
| 10X Single Cell RNA Library (2-7 samples, each) | $2,190 |
| 10X Single Cell RNA Library (8 samples submitted at once, each) | $1,960 |
| 10X Single Cell RNA, poly-A, multiplexed (4-plex/5k ea) | $3,700 |
| 10X GEM-X Flex RNA Sample Fixation, Set up fee | $296 |
| 10X GEM-X Flex RNA Sample Fixation, each | $148 |
| 10x GEM-X Flex RNA, 4 channels (4 samples) | $8,750 |
| 10x GEM-X Flex RNA, 1 channel (up to 4 samples) | $4,790 |
| 10x GEM-X Flex RNA, 1 channel (up to 16 lib) | $11,690 |
| 10x GEM-X Flex RNA, 4 channels (up to 64 lib) | $45,310 |
| 10X Single Nuclei ATAC Library, each | $2,730 |
| 10X Single Nuclei ATAC Library (2-7 samples, each) | $2,390 |
| 10X Single Nuclei ATAC Library (8 samples submitted at once, each) | $2,000 |
| 10X Single Nuclei ATAC+RNA Combined Library, each | $4,490 |
| 10X Single Nuclei ATAC+RNA Combined Library (2-7 samples, each) | $4,030 |
| 10X Single Nuclei ATAC+RNA Combined Library (8 samples at once, each) | $3,640 |
| 10X Visium Spatial library (cDNA & kit provided) | $131 |
ILLUMINA NOVASEQ X PLUS
| Service | Price |
| 1.5B Flowcell= 750M Read-Pairs Per Lane | |
| Nova X 1.5B paired-reads 2×150-cycle, per lane | $1,430 |
| 1.5B Flowcell= 750M Single-Reads Per Lane | |
| Nova X 1.5B single-reads 100-cycle, per lane | $1,230 |
| 10B Flowcell= 1 Billion Read-Pairs Per Lane | |
| Nova X 10B paired-reads 2×150-cycle, per lane | $2,110 |
| Nova X 10B paired-reads 2×150-cycle, per lane (8 or more lanes) | $1,650 |
| 10B Flowcell= 1.2 Billion Single-Reads Per Lane | |
| Nova X 10B single-reads 100-cycle, per lane | $1,570 |
| Nova X 10B single-reads 100-cycle, per lane (8 or more lanes) | $1,330 |
| 25B Flowcell= 3.4 Billion Read-Pairs Per Lane | |
| Nova X 25B paired-reads 2×150-cycle, per lane | $3,240 |
| Nova X 25B paired-reads 2×150-cycle, per lane (8 or more lanes) | $3,010 |
ILLUMINA NOVASEQ-STANDARD
| Service | Price |
| SP flowcell (per lane) (750+ million reads) | |
| NovaSeq SP-per lane 2x250nt Paired-Read | $3,240 |
ILLUMINA MISEQ & MISEQ i100
| Service | Price |
| i100 titration of pools (26nt + index reads) | $382 |
| i100 5M 2x150nt (5 million read-pairs) | $492 |
| i100 5M 2x300nt (5 million read-pairs) | $582 |
| i100 25M Single Read 100nt (25 million single reads) | $773 |
| i100 25M 2x150nt (25 million read-pairs) | $1,210 |
| i100 25M 2x300nt (25 million read-pairs) | $1,450 |
| MiSeq – 2x150nt PE NANO v2 | $577 |
| MiSeq – 2x250nt PE NANO v2 | $635 |
| MiSeq – 2x250nt PE v2 | $1,770 |
| MiSeq – 2x250nt PE v3 | $2,240 |
| MiSeq – 2x300nt PE v3 | $2,140 |
PACBIO LIBRARIES AND REVIO SEQUENCING
| Service | Price |
| Generation of full-length 16S amplicons, each | $35 |
| Generation of full-length 16S amplicons, >48 | $26 |
| Generation of full-length 16S amplicons, 2-step, each | $37 |
| Generation of full-length 16S amplicons, 2-step, >48 | $28 |
| SMRTBell Library Construction from Amplicons | $293 |
| FemtoPulse (1-11 samples or libraries) | $159 |
| HMW DNA Size Selection (1-2) | $256 |
| SMRTBell Library Construction, HiFi | $672 |
| SMRTBell Library Construction, barcoded, 2 | $445 |
| SMRTBell Library Construction, barcoded, 3-11 | $332 |
| SMRTBell Library Construction, barcoded, 12+ | $258 |
| SMRTBell Library Construction, UltraLow | $641 |
| SMRTBell Library Construction, UltraLow barcoded, 2-11 | $487 |
| SMRTBell Library Construction, UltraLow barcoded, 12+ | $346 |
| IsoSeq Library Construction | $525 |
| IsoSeq Library Construction, 2-3 | $321 |
| IsoSeq Library Construction, 4-11 | $223 |
| IsoSeq Library Construction, 12+ | $162 |
| 16S Kinnex Library Construction | $828 |
| 16S Kinnex Library Construction, 2+ | $673 |
| RNA Kinnex Library Construction | $995 |
| RNA Kinnex Library Construction, 2-3 | $577 |
| RNA Kinnex Library Construction, 4-11 | $352 |
| RNA Kinnex Library Construction, 12+ | $221 |
| RNA Kinnex Library Construction, 48+ | $203 |
| PacBio Multiplexed Microbial Library (2-3) | $333 |
| PacBio Multiplexed Microbial Library (4-7) | $158 |
| PacBio Multiplexed Microbial Library (8-19) | $123 |
| PacBio Multiplexed Microbial Library (20-47) | $93 |
| PacBio Multiplexed Microbial Library (48-95) | $78 |
| PacBio Multiplexed Microbial Library (96+) | $68 |
| Sequencing on Revio SMRTcell | $2,180 |
| PACBio 8M CCS demultiplexing fee | $269 |
PACBIO DISCOUNTED SEQUENCING
| Sequencing on Revio SMRTcell, 4+ | $2,060 |
OXFORD NANOPORE GRIDION
| Service | Price |
| Construction of Oxford-Nanopore 1D libraries, standard | $652 |
| Construction of Oxford-Nanopore 1D libraries, barcoded (2+) | $489 |
| Construction of Oxford-Nanopore Direct RNA libraries | $386 |
| Construction of Oxford-Nanopore Direct RNA libraries (2-6) | $308 |
| Oxford-Nanopore GridION flowcell, each | $1,270 |
SANGER CORE SEQUENCING
| Service | Price |
| Sanger – High-Throughput Ready to Load (96-well Plate) – no clean up | $96 |
| Sanger – High-Throughput Ready to Load (96-well Plate) – with clean up | $228 |
| Sanger – High-Throughput Sequencing (96-well plate) | $444 |
| Sanger – Low-Throughput Sequencing (One Sample) | $5.90 |
| Sanger – Low-Throughput Ready to Load (One Sample, includes clean-up) | $3.20 |
| Fragment Analysis – High-Throughput (96-well Plate) | $119 |
| Fragment Analysis – Low-Throughput (One Sample) | $4.10 |
Please visit the Sanger Sequencing and Fragment Analysis page for details regarding each service option. Please contact Core staff at 217-265-6814 or dna-seq@illinois.edu for any additional questions on Sanger Sequencing or Fragment Analysis.
This is an abridged list of our service prices and subject to change at any time. For further information please contact Dr. Alvaro Hernandez, Director of DNA Services ( aghernan@illinois.edu ) at 217-244-3480 or Chris Wright, Assistant Director of DNA Services ( clwright@illinois.edu ) at 217-333-4372 to discuss ways the staff can be of assistance in achieving your project goals or to receive a quote for your project, submission forms, grant support or any other information needed.
External users are charged an additional 31.7% overhead fee, which is capped at 20% for those users from State of Illinois subsidized universities and government agencies. For further information on the contract, please contact Dr. Marcello Rubessa (rubessa@illinois.edu), Associate Director of the Roy J. Carver Biotechnology Center.
All scientific work performed by the Roy J. Carver Biotechnology Center (CBC)is required to be acknowledged in scholarly publications, posters, and presentations. Proper recognition allows us to measure the impact of our work and supports our initiatives in obtaining sponsored funding. In addition, any CBC personnel who make a substantial intellectual or experimental contribution are deserving of further recognition as co-author.
Contact
DNA Services
Director: Alvaro G. Hernandez Ph. D.
University of Illinois at Urbana-Champaign
Roy J. Carver Biotechnology Center
1201 W. Gregory Drive
329 Edward R. Madigan Laboratory (ERML)
Urbana, IL 61801
(217) 244-3480