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Cytometry and Microscopy to Omics
Mayandi Sivaguru, Ph.D - Director
231 Edward R. Madigan Laboratory,
1201 W. Gregory Drive, Urbana, IL 61801
Phone: (217) 333-1214   
Email: sivaguru@illinois.edu

 

The Cytometric Bead Array System

Multiplexed Bead-Based Immunoassays

Picture obtained from BD Cytometric Bead Array – Multiplexed Bead-Based Immunoassays

If you are looking for a good alternative to enzyme-linked immunosorbent assays to efficiently and simultaneously measure different panels of analytes (chemokines, cytokines, phosphoproteins, growth factors, human immunoglobulins etc.) in a single microplate well, then multiplex bead assay is your choice.

Our Main Facility is equipped with a Bio-Rad Luminex Cytometric Bead Analyzer, which can perform up to 40 assays (each bead is infused with fluorescent dyes in different ratios allowing for emission of different amounts of photons depending upon the amount of binding) in a single well, increasing assay flexibility as it can be used to perform immunoassays, nucleic acid research, enzymatic research, and receptor-ligand studies.Table
Picture obtained from BIO-RAD – Multiplex Immunoassays

Multiple kits are available on the market, preconfigured or configurable kits (build your own multiplexes).
BD CBA Flex Sets - BD Biosciences
BD CBA Kits - BD Biosciences
Immunoassays and Multiplex kits - EMD Millipore
Multiplex Kits - ThermoFisher
Multiplex Cytokine Assay Kits - R&D systems (bio-techne brand)
Bio-Plex Suspension Array System - BIO-RAD

Luminex Assays and Luminex High Performance Assays - for simultaneously detecting and quantifying multiple target analytes in qualified complex sample types (accurate and reproducible results for every target analyte).

xMAP Technology Analyte Menu 

xMAP Technology Products and Services Catalog

A paper to read if you want to know more:
Bead-Based Multianalyte Flow Immunoassays - The Cytometric Bead Array System

                                        Scheduling

Please contact us to schedule an appointment or to discuss a future project: flowcyt@illinois.edu, (217) 244-0559.