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Metabolomics Center
Dr. Zhong (Lucas) Li, Director
264 Burrill Hall, MC-120
407 S Goodwin 
Urbana, IL 61801
Phone: (217) 333-5939    
Email: lucasli@illinois.edu

SERVICES/EQUIPMENT

Services

The primary goal of the Metabolomics Lab is to identify, profile, and quantify small molecules (metabolites) from various resources (plant, animal, human) with origins such as cell, cell medium, tissue, urine, blood, serum, plasma, feces, etc. Multiple complementary analytical methods are utilized primarily with mass spectrometry. We provide support for targeted metabolite profiling (alcohols, alkaloids, amine and polyamines, amino acids, fatty acids up to C30, lipids, nucleotides, organic acids, phenolics, phthalates, sterols, steroids/hormones, sugars and sugar phosphates, a wide range of drug compounds, and many more emerging metabolites) and untargeted metabolite profiling. We help execute the projects from the start to the end: sample preparation protocol development --> instrument method development --> data acquisition on the instrument --> data analysis (including metabolomics related statistical analysis if needed).

The Metabolomics Lab at University of Illinois has a suite of instruments (GC/MS and LC/MS) and related software package for metabolite analysis. These mass spectrometry-based setups can achieve highly sensitive quantitative analysis, very accurate metabolite identification, and much more. The instrument platform is well suited to perform targeted metabolite analysis (quantitatively and qualitatively) and untargeted metabolite profiling for samples up to BSL-2.

Users (both on- and off-campus) are encouraged to discuss their projects with the center staff before using the services and instrumentation in their experimentation.

For off-campus projects, please fill Off-Campus User Form. If the project is exceeding $5,000, a Technical Testing Services Agreement is required.

For additional information please contact Dr.Lucas Li(lucasli@illinois.edu)

Services:

  • GC/MS based metabolite profiling (targeted)
  • LC/MS based metabolite profiling (untargeted)
  • LC/MS/MS based lipid profiling
  • Quantitative analysis via GC/MS (full scan or SIM)
  • Quantitative analysis via LC/MS/MS (MRM)
  • Qualitative analysis via GC/MS or LC/MS or LC/MS/MS
  • High resolution LC/MS (or LC/MS/MS) analysis

 

Equipment

Thermo LC/MS---Altis Triple Quadrupole Mass spectrometer

Altis Triple quadrupole Mass Spec

 

The TSQ Altis MS is designed for stable, reliable and consistent performance. It has the following features:

  • Mass range: m/z 5 ~ m/z 2,000
  • Equipped with ESI source
  • Operated with Tracefinder software
  • Connected with Vanquish UHPLC system
  • Ultrafast selected reaction monitoring (SRM) increases the amount of molecular quantifications in less time

For: High-throughput quantitative analysis

Thermo LC/MS --- Q-Exactive Mass Spectrometer

Thermo Q-Exactive Mass Spectrometer

This Hybrid Quadrupole-Orbitrap system combines quadruple precursor ion selection with high-resolution,
accurate-mass (HRAM) Orbitrap detection to help identify and confirm more metabolites. It has the following
features with ESI source:

  • Better than 3ppm mass accuracy in full-scan with external calibration
  • Resolving power up to 140,000 FWHM
  • Extended mass range of m/z 50 - 6,000
  • Dedicated data analysis software Compound Discoverer and LipidSearch for metabolite information mining including lipid profiling.
  • Connected with Ultimate 3000 HPLC along with diode array detector (DAD)

For: Untargeted metabolite profiling
Lipidomics analysis

Sciex LC/MS---5500 QTrap Mass Spectrometer

Sciex LC-MS5500 QTrap MS

A benchtop hybrid triple Quadrupole-linear accelerator trap mass spectrometer designed for LC/MS/MS analysis. Available Turbo V™ Source includes ESI, and APCI. It has the following features:

  • Scan modes include: full scan and selected ion scan for both Q1 and Q3, Product Ion Scan, Precursor Ion Scan, Neutral Loss Scan, Multiple Reaction Monitoring (MRM), Enhanced MS Scan, Enhanced Product Ion Scan, Enhanced Resolution Scan, and MS3 scan
  • Mass range of m/z 5 ~ m/z 1,250
  • Connected with Agilent 1200 HPLC

For: Superior quantitative analysis

Sciex LC/MS---6500+ Triple Quadrupole Mass Spectrometer

Sciex LC-MS6500 Triple Quadrupole MS

A benchtop triple Quadrupole mass spectrometer designed for LC/MS/MS analysis. Available IonDrive Turbo V Source includes ESI, and APCI. It has the following features:

  • Scan modes include: full scan and selected ion scan for both Q1 and Q3, Product Ion Scan, Precursor Ion Scan, Neutral Loss Scan, Multiple Reaction Monitoring (MRM)
  • Mass range of m/z 5 ~ m/z 2,000
  • Up to 6 orders of linear dynamic range
  • Increased throughput with polarity switching speeds of 5 msec
  • Connected with Agilent 1260 infinite II HPLC

For: Utmost quantitative analysis

Agilent GC/MS

Agilent GC-MS Utmost quantitative analysis

A benchtop mass spectrometer designed for small metabolite analysis. It has the following features:

  •  
  • Equipped with EI and CI sources
  • 7890B GC with 5977A Extractor XL MS
  • With commercial Wiley and NIST libraries
  • Mass range of m/z 2 ~ m/z 800
  • Operated with MassHunter Software

For: Utmost quantitative analysis

 

Agilent GC/MS

Agilent GC-MS Targeted metabolite Profiling

A benchtop mass spectrometer designed for small metabolite analysis. It has the following features:

  • Equipped with EI and CI sources
  • 7890A GC with 5975C MS
  • With commercial Wiley and NIST libraries
  • Mass range of m/z 2 ~ m/z 800

For: Targeted metabolite profiling analysis

Agilent GC/MS

Agilent GC-MS qualitative analysis

A benchtop mass spectrometer designed for small metabolite analysis. It has the following features:

  • Equipped with EI and CI sources
  • 6890N GC with 5973 MS With commercial Wiley and NIST libraries
  • Mass range of m/z 2 ~ m/z 800
For: Qualitative analysis

SpectraMax M2 Multi-detection reader

Spectra Max

  • Supports 6- to 384-well microplate and cuvette reading
  • Absorbance (200-1,000 nm)
  • Luminescence
  • Fluorescence (excitation: 250-850 nm, emission: 360-850 nm)