Nucleic Acid/Protein Quantification
Qubit Nanodrop
Accurately determining the concentration of your samples is vital for many downstream applications. We offer both a Nanodrop 1000 Spectrophotometer and a Qubit 2.0 Fluorometer for general usage. The Nanodrop is available as a self-service model with a 1 year contract. Use of the Qubit can be self service (user provides all reagents) with a 1 year contract or by sample drop-off and our staff will do the measurements.
For pricing, please see our Pricing Page
Forms:
Yearly Nanodrop form
Yearly Qubit form
Qubit Sample Submission form
Qubit Submissions:
-We recommend samples be submitted in individual tubes, with each tube labeled with sample name and PI’s initials.
-Submission form must be completed and dropped off with samples OR sent by email (see contacts). If you are submitting the form by email, please have your PI send an email confirming the account number.
-Please contact the lab before bringing your samples so we can ensure that they are placed immediately into the -80C freezer.
-Samples must be brought to the lab on ice.
Results:
FAQs:
Q: How long will it take to get my Qubit results (for drop off samples)?
A: The normal turnaround time is 1-2 business days.
Q: Should I use the Nanodrop or the Qubit to quantitate my samples?
A: Each technology has pros and cons:
Quibit:
- Accurate at very low concentrations
- Measures only the nucleic acids in a sample and is specific for RNA or DNA
- Does not provide 260/280 or 260/230 ratios
Nanodrop:
- Accurate at higher concentrations (above 30ng/ul)
- May take contaminants into account when quantitating nucleic acid and does not differentiate between RNA and DNA
- Provides 260/230 and 260/280 ratios
While these instruments will allow you to measure the quantity and purity of nucleic acid or protein, they will not give data on the quality of each sample. For that, please refer to the Bioanalyzer page
Contacts:
-Please contact Hyemi Kim (hyemik2@illinois.edu) with any additional questions.
-Lab address/shipping information:
University of Illinois
356 Edward R. Madigan Laboratory
1201 W. Gregory Dr.
Urbana, IL 61801
-Phone number:
217-244-3929
For further information and service fees, contact: Mary Majewski or Mark Band in the Functional Genomics Lab at (217)244-3929