Protein Sciences Facility Pricing
Prior to project initiation, a meeting is required with CBC staff to discuss each project. If mutually agreed that the CBC can support the project, we will deliver a quote outlining the approach, timeline, and cost for the agreed service(s). As this is cutting-edge science, we cannot guarantee success, in all cases the user will be billed for cost and labor for CBC effort per our fee structure. After acceptance of the quote, the user will fill out and return the sample submission form provided with the quote, and also found on each CBC facility’s website. All samples are assumed to be safe, and no greater than BSL1, unless otherwise agreed upon in advance. The user must diligently and transparently followed all University of Illinois sample acquisition, movement, and safety protocols.
Prices effective July 1, 2022
Preparative Liquid Chromatography |
|
FPLC Buffer Preparation Fraction Collection |
$194.00/injection $25.00-50.00/analysis $22.00/plate |
Self Service |
|
Typhoon 9400 Imaging (half hour minimum) |
$89.00/half hour |
Intact Mass Analysis with Advion TriVersa and Fusion |
$132.00/hour + $5.00/nozzle |
Intact Mass Analysis with Advion TriVersa and UHMR |
$142.00/hour + $5.00/nozzle |
Enzymatic Digestion |
|
Trypsin (default unless otherwise noted) |
$53.00/sample |
Glu-C |
$100.00/sample |
Lys-C |
$53.00/sample |
LysC + Trypsin sequential digest |
$100.00/sample |
Other proteases (chymotrypsin, thermolysin, etc.) |
$100.00/sample |
Additional Proteomic Sample Preparation |
|
Protein Extraction |
$62.00/sample |
Reduction & Alkylation |
$41.00/sample |
Sample Desalting (StageTip) |
$38.00/sample |
Detergent Removal |
$50.00/sample |
Protein Precipitation |
$50.00/sample |
C18 Column (Sep-Pak) |
$60.00/sample |
Buffer Exchange (Spin column) |
$38.00/sample |
Phosphorylation Enrichment (Fe-IMAC or TiO2) |
$74.00/sample |
Protein Concentration Measurement (BCA Assay) |
$65.00/plate |
Mass Spectrometry |
|
LC-MS/MS (45 min rapid analysis) |
$124.00/sample |
LC-MS/MS (60 min standard analysis) |
$165.00/sample |
LC-MS/MS (90 min analysis) |
$186.00/sample |
LC-MS/MS (120 min extended analysis) |
$207.00/sample |
LC-MS/MS (180 min analysis) |
$248.00/sample |
In-gel bundle for on-campus projects (CEM digest + rapid LC-MS/MS) |
$158.00/sample |
Quantitative Proteomic Analysis Label-free Isotopic label (iTRAQ, TMT, dimethyl labeling, etc.) |
inquire for quote |
Intact protein analysis* |
$132.00/sample |
Lyophilization |
|
5 mL and under |
$54.00/sample |
5 mL to 200 mL |
$60.00/sample |
Additional Data Analysis |
$52.00/hour |
Custom Database or Modification Added to Mascot Server |
$13.00/addition |
*Prior consultation recommended
External users are charged an additional 31.7% overhead fee, which is capped at 20% for those users from State of Illinois subsidized universities and government agencies. Off-campus projects require an off-campus user testing agreement, to be provided by the facility, and if over $10,000 a technical testing agreement. For further information contact Dr. Mark A. Mikel (217-244-0144; mmikel@illinois.edu ), Associate Director of the Roy J. Carver Biotechnology Center.
All scientific work performed by the Roy J. Carver Biotechnology Center (CBC) is required to be acknowledged in scholarly publications, posters, and presentations. Proper recognition allows us to measure the impact of our work and supports our initiatives in obtaining sponsored funding. In addition, any CBC personnel who make a substantial intellectual or experimental contribution are deserving of further recognition as co-author.