Immunophenotyping
Direct staining
Fluorescent probe attached to primary antibody:
Protocols on the WEB
Direct Immunofluorescence Staining for CMtO from AbDSerotech
Direct Flow Staining Protocol from Abcam
Indirect staining
Fluorescent probe attached to a secondary antibody:
Protocols on the WEB
Indirect Immunofluorescence Staining for CMtO from AbDSerotech
Indirect Flow Staining Protocol from Abcam
Avidin-Biotin Method
Biotynylated antibody can bind many avidins (streptavidins) - fluorochromes complex to improve sensitivity of assay:
Zenon® Labeling Technology
Zenon technology is based on the complexation of primary antibodies with dye– or enzyme–labeled Fab fragments of secondary antibodies directed against their Fc regions.
Picture obtained from invitrogen webpage about Zenon® Labeling Technology
General Staining techniques
Surface Staining Protocol from University of Utah Flow Cytometry Facility
Vendors
Biocampare Antibodies
Biolegend
Thermo Fisher Antibodies
BD Biosciences Multicolor
EMD Millipore Antibodies
affymetryx eBioscience Immunology
Abcam
All work performed by the Roy J. Carver Biotechnology Center (CBC) should be acknowledged in scholarly publications, posters, and presentations. Proper recognition allows us to measure the impact of our work and supports our initiatives in obtaining sponsored funding. In addition, any CBC personnel who make a substantial intellectual or experimental contribution are deserving of further recognition as co-author.