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Roy J. Carver Biotechnology Center

HTDNA Group

DNA

MiSeq Flowcell

At Computer

Magnetic Plate

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IllinoisThe primary mission of the DNA Services Lab is to provide outstanding service and state-of-the-art instrumentation to support basic, environmental, agricultural, and translational medical research for our campus investigators and external academic and corporate collaborators.

 

Instruments


Applications

Instrumentation

*We are an Illumina Certified Sequence Provider

NovaSeq 6000

Illumina NovaSeq 6000

This is the latest instrument from Illumina for ultra-high throughput sequencing of genomic DNA, RNA-Seq, bisulfite-treated DNA, targeted and mate-pair libraries and any samples that need high depth.

 

 

NovaSeq Sequencing Options

Flowcell Read Type and Length Price Per Lane # of Single-Reads # of Paired-Reads Gb/lane
Sp Single-reads,100bp $1,730 400-500 million   40-50
  Paired-reads, 2x150bp $2,400   800 million+ 120
  Paired-reads, 2x250bp $3,260   800 million+ 200
           
S1 Single-reads,100bp $3,400 800 million+   80
  Paired-reads, 2x100bp $4,340   1.5 billion+ 160
  Paired-reads, 2x150bp $4,700   1.5 billion+ 240
           
S2 Single-reads, 100bp $4770 1.5 billion+   165
           
S4 Paired-reads, 2x150bp $5930   5-6 billion 750-850

 

PacBio

PacBio Sequel IIe

This is the latest instrument from PacBio, it produces the longest reads with the highest accuracy versus previous PacBio instruments. It is the instrument of choice for assembly of complex genomes. Also, it is used for characterization of full-length 16S and other amplicons and of full-length transcriptomes (IsoSeq).

One SMRT Cell 8M produces

  • 20-30 Gbases of HiFi error-corrected reads with fragments 10-25kb
  • 150-250+ Gbases of raw reads with fragments 10kb to 50kb
  • 2-4 million error corrected cDNA or amplicon reads.
 
MiSeq

Illlumina MiSeq

 For sequencing libraries from amplicons (ie: 16S, 18S, ITS, etc), genomic DNA, small RNAs, RNA-Seq, ChIP-Seq, etc.

  • Single- or paired-reads, 50bp to 300bp in length
  • V2 Nano run produces 500k to 2 million paired-reads
  • V2 bulk run produces 8 to 20 million paired-reads
  • V3 run produces 10 to 40 million paired-reads
  • The flowcell contains 1 lane
 
GridION

Oxford Nanopore GridION

Long sequencing reads from genomic DNA, RNA, cDNA, 16S or other amplicons.

  • One flowcell produces from 1 to 9 million reads depending on the type of sample
  • Reads are typically from 5kb to 40kb and with some reads from HMW genomic DNA reaching up to 250kb
  • Each flowcell runs one or multiple samples
 
10x

10x Genomics

The 10x Chromium system produces libraries for 3' transcriptome sequencing from single-cells, ATAC-seq and multiomics (RNAseq + ATACseq).

  • Libraries constructed on 10x are fully compatible with Illumina sequencing.
  • Single-cell libraries can be constructed from 500 to 10,000 cells per library.
  • Single-cell libraries are sequenced for 28x100nt or 28x150nt on the NovaSeq, with a minimum of 50k reads per cell recommended.
  • 10x Visium Spatial gene expression libraries are also available.