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Pricing

Prior to project initiation, a meeting is required with CBC staff to discuss each project. If mutually agreed that the CBC can support the project, we will deliver a quote outlining the approach, timeline, and cost for the agreed service(s). As this is cutting-edge science, we cannot guarantee success, in all cases the user will be billed for cost and labor for CBC effort per our fee structure. After acceptance of the quote, the user will fill out and return the sample submission form provided with the quote, and also found on each CBC facility’s website. All samples are assumed to be safe, and no greater than BSL1, unless otherwise agreed upon in advance. The user must diligently and transparently follow all University of Illinois sample acquisition, movement, and safety protocols.

All pricing is updated, effective April 1st, 2020. Listed rates do not include the off-campus overhead (see bottom of page for overhead details).

Illumina NovaSeq 6000Ultra high-throughput sequencing of mRNA, genomic DNA, ChIP-DNA, small RNAs, bisulfite-treated DNA, single-cell transcriptomics, etc. For descriptions of library construction requirements and sequencing platform metrics, please visit our Sample Submission Page.

PacBio Sequel II: accurate long reads for de novo genome assembly and sequencing of long amplicons or cDNA.

Illumina Library Prep Services

Price

Sample or library quality control (QC)

 

Quality Check (QC) - one library (Qubit, Fragment Analyzer & qPCR)

$92

Quality Check  (QC) - 2-8 libraries (Qubit, Fragment Analyzer & qPCR)

$176

Sample Purification (eGel or SPRI bead. Does not include QC)

$89

Sample QC: Qubit & Fragment Analyzer (Minimum charge is for 8 samples)

$12

AATI Fragment Analyzer service (up to 96 samples or libraries)

$298

AATI Fragment Analyzer service (1-12 samples or libraries)

$65

HMW DNA Size Selection (40kb+, 1 or 2 samples)

$108

Library Construction

 

Shotgun genomic DNA library prep, each

$172

Shotgun genomic DNA prep in 96-well plate, ≥24

$93

Shotgun genomic DNA prep in 96-well plate, ≥48

$80

 

RNA-Seq library, PolyA selected RNA, each

$150

RNA-Seq library, PolyA selected RNA (≥24 on robot, full QC)

$115

RNA-Seq library, PolyA selected RNA (≥48 on robot, full QC)

$100

RNA-Seq library, PolyA selected RNA (≥96 on robot, full QC)

$89

 

RNA-Seq library, rRNA depleted, each

 

$248

RNA-Seq library, rRNA depleted (≥24 on robot, full QC)

$213

RNA-Seq library, rRNA depleted (≥48 on robot, full QC)

$194

RNA-Seq library, rRNA depleted (≥96 on robot, full QC)

   $173

 

Small RNA libraries, each

 

$170

Small RNA libraries (≥24 on robot)

$159

Small RNA libraries (≥48 on robot)

   $148

Small RNA libraries (≥96 on robot)

   $130

 

Mate-Pair libraries, standard (bacterial, small genomes)

 

$820

Mate-Pair libraries, enriched (> 100MB genomes)

$1,090

 

Exome Capture libraries

 

$250

Exome Capture libraries (kit provided)

$125

Exome Capture libraries (≥48 on robot)

$188

 

Bisulfite, reduced representation DNA libraries, each

 

$152

Bisulfite, reduced representation DNA libraries (≥48 on robot)

$127

Bisulfite, genomic DNA libraries

$138

Bisulfite, genomic DNA libraries (≥48 on robot)

$120

 

MiSeq FGx amplicon preparation, each

 

$168

MiSeq FGx amplicon preparation, each, > 32 

   $138

 

10x DNA Library Construction (1 library by itself, faster turnaround time)

 

$1,100

10x DNA Library Construction (1-7 samples from one or different users, each)

$825

10x DNA Library Construction (8 samples submitted at once, each)

$698

 

10x Single Cell RNA Library (1 library by itself, faster turnaround time)

 

$2,360

10x Single Cell RNA Library (2-7 samples, each)

$2,030

10x Single Cell RNA Library (8 samples submitted at once, each)

$1,860

 

10x  Visium Spatial Transcriptomics Library (each, kit provided)

 

$30

 

Fluidigm PCR amplification, 1 plate (up to 48 samples)

 

$1,550

Fluidigm Primer testing (1-3, each)

$100

Fluidigm Primer testing (4+, each)

$50

Illumina NovaSeq 6000

(we are an Illumina Certified Service Provider)

 

SP flowcell    (per lane)

 
          Single-Reads,  100bp        (350+ million reads) $1,720
          Paired-Reads, 2x150bp    (750+ million reads) $2,380
          Paired-Reads, 2x250bp    (750+ million reads) $3,350

S1 flowcell    (per lane)

 
          Single-Reads,  100bp        (750+ million reads)

$3,370

          Paired-Reads, 2x100bp     (1.5+ billion reads) $4,280
          Paired-Reads, 2x150bp     (1.5+ billion reads) $4,600

S2 flowcell    (per lane)

 
          Single-Reads,  100bp    (1.5+ billion reads) $5,350

S4 flowcell    (per lane)

 
          Paired-Reads, 2x150bp   (5-6 billion reads) $6,300
 

 

Illumina MiSeq v3

 

MiSeq - titration of library pools (26nt + index read)

          $299

MiSeq - 1x150nt SR v3

$1,380

MiSeq - 2x150nt PE NANO v2

$675

MiSeq - 2x150nt PE v2

$1,600

MiSeq - 2x250nt PE NANO v2

$737

MiSeq - 2x250nt PE v2

$1,700

MiSeq - 2x250nt PE v3

$2,070

MiSeq - 1x300nt SR v2

$1,600

MiSeq - 2x300nt PE v3

$2,160

MiSeq -FGx run

$2,210

 

PacBio Sequel II

 
Generation of full-length 16S amplicons, each $20
Generation of full-length 16S amplicons, >49 $15
SMRTBell Library Construction from Amplicons $423
SMRTBell Library Construction, CLR $699
SMRTBell Library Construction, HiFi $699
SMRTBell Library Construction, barcoded, 2+ $625
SMRTBell Library Construction, barcoded, 12 $526
Iso-Seq Library Construction  $640
Sequencing on SMRT 8M cell, Sequel II, 10h movie $1,860
Sequencing on SMRT 8M cell, Sequel II, 30h movie $1,950

Oxford-Nanopore GridION

 
Construction of Oxford-Nanopore 1D libraries, low input $480
Construction of Oxford-Nanopore 1D libraries, standard $407
Construction of Oxford-Nanopore 1D libraries, barcoded $350
Construction of Oxford-Nanopore cDNA libraries  $392
Construction of Oxford-Nanopore Direct RNA libraries  $305
Construction of Oxford-Nanopore Rapid DNA libraries  $277
**Additional discount available for multiple libraries, please contact us for project pricing.  
Oxford-Nanopore GridION flowcell, each $790

Core Sequencing

Price

Sanger - High-Throughput Ready to Load (96-well Plate) - no clean up

$64

Sanger - High-Throughput Ready to Load (96-well Plate) - clean up

$148

Sanger - High-Throughput Sequencing (96-well plate)

$390

Sanger - Low-Throughput Sequencing (One Sample)

$4.90

Sanger - Low-Throughput Ready to Load (One Sample, includes clean-up)

$2.10

Fragment Analysis - High-Throughput (96-well Plate)

$85

Fragment Analysis - Low-Throughput (One Sample)

$2.70

Please visit the Sanger Sequencing and Fragment Analysis page for details regarding each service option. Please contact Core staff at 217-265-6814 or dna-seq@illinois.edu for any additional questions on Sanger Sequencing or Fragment Analysis.

This is an abridged list of our service prices and subject to change at any time.  For further information please contact Dr. Alvaro Hernandez, Director of DNA Services ( aghernan@illinois.edu ) at 217-244-3480 or Chris Wright, Assistant Director of DNA Services ( clwright@illinois.edu ) at 217-333-4372 to discuss ways the staff can be of assistance in achieving your project goals or to receive a quote for your project, submission forms, grant support or any other information needed.

Facility users not within the State of Illinois are subject to a 31.9% indirect cost rate. State of Illinois institutions are subject to a 31.9% indirect cost rate for Federally-funded projects and 10% indirect cost rate for non-Federally funded projects. For off-campus projects exceeding $10,000, a contract must be negotiated through University of Illinois Grants and Contracts office.  For further information on the contract, please contact Dr. Mark A. Mikel (217-244-0144; mmikel@illinois.edu ), Associate Director of the Roy J. Carver Biotechnology Center.

All scientific work performed by the Roy J. Carver Biotechnology Center (CBC)is required to be acknowledged in scholarly publications, posters, and presentations.  Proper recognition allows us to measure the impact of our work and supports our initiatives in obtaining sponsored funding. In addition, any CBC personnel who make a substantial intellectual or experimental contribution are deserving of further recognition as co-author.