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Pricing

Prior to project initiation, a meeting is required with CBC staff to discuss each project. If mutually agreed that the CBC can support the project, we will deliver a quote outlining the approach, timeline, and cost for the agreed service(s). As this is cutting-edge science, we cannot guarantee success. In all cases the user will be billed for cost and labor for CBC effort per our fee structure. After acceptance of the quote, the user will fill out and return the sample submission form provided with the quote, and also found on each CBC facility’s website. All samples are assumed to be safe, and no greater than BSL1, unless otherwise agreed upon in advance. The user must diligently and transparently follow all University of Illinois sample acquisition, movement, and safety protocols.

All pricing is updated, effective July 1st, 2023. Listed rates do not include the off-campus overhead (see bottom of page for overhead details).

Illumina NovaSeq X Plus and 6000Ultra high-throughput sequencing of mRNA, genomic DNA, ChIP-DNA, small RNAs, bisulfite-treated DNA, single-cell transcriptomics, etc. For descriptions of library construction requirements and sequencing platform metrics, please visit our Sample Submission Page.

PacBio Sequel IIe: accurate long reads for de novo genome assembly and sequencing of long amplicons or cDNA.

Illumina Library Prep Services

Price

Sample or library quality control (QC)

 

Hourly rate, cell test counts, other (per hour) $90

Quality Check (QC) - one library (Qubit, Fragment Analyzer & qPCR)

$109

Quality Check  (QC) - 2-8 libraries (Qubit, Fragment Analyzer & qPCR)

$258

Sample Purification (eGel or SPRI bead. Does not include QC)

$161

Sample QC: Qubit & Fragment Analyzer (Minimum charge is for 8 samples)

$14

AATI Fragment Analyzer service (up to 96 samples or libraries)

$417

AATI Fragment Analyzer service (1-12 samples or libraries)

$97

AATI FemtoPulse (1-11 samples or libraries)

$144

HMW DNA Size Selection (40kb+, 1 or 2 samples)

$247
Covaris Annual User fee $200

Library Construction

 

BULK ATAC-Seq ActiveMotif library, <24 

$191

Shotgun genomic PCR-free prep, each

$172

Shotgun genomic DNA library prep, each (1-11)

$147

Shotgun genomic DNA library prep, each (12-23)

$106

Shotgun genomic DNA library prep, each (24-47)

$87

Shotgun genomic DNA library prep, each (48-95)

$72

Shotgun genomic DNA library prep, each (96+)

$61

SkimSeq Libraries, each (48+)

$23
SkimSeq Libraries, each (48+; plus QC) $27

 

RNA-Seq library, PolyA selected RNA, each

 

$119

RNA-Seq library, PolyA selected RNA (≥12 on robot, full QC)

$101

RNA-Seq library, PolyA selected RNA (≥24 on robot, full QC)

$86

RNA-Seq library, PolyA selected RNA (≥48 on robot, full QC)

$79

RNA-Seq library, PolyA selected RNA (≥96 on robot, full QC)

$71

 

RNA-Seq library, rRNA depleted, each

 

$171

RNA-Seq library, rRNA depleted (≥12 on robot, full QC)

$152

RNA-Seq library, rRNA depleted (≥24 on robot, full QC)

$125

RNA-Seq library, rRNA depleted (≥48 on robot, full QC)

$118

RNA-Seq library, rRNA depleted (≥96 on robot, full QC)

   $110

 

Small RNA libraries, each

 

$163

Small RNA libraries (≥24 on robot)

$131

Small RNA libraries (≥48 on robot) $123
Small RNA libraries (≥96 on robot)    $116

Bacterial Small RNA libraries (≥24 on robot)

$170

Bacterial Small RNA libraries (≥48 on robot)

$163

Bacterial Small RNA libraries (≥96 on robot)

$155

 

Exome Capture libraries

 

$189

Exome Capture libraries (>48 on robot)

$157

 

Bisulfite, reduced representation DNA libraries, each

$140

Bisulfite, reduced representation DNA libraries (≥24 on robot)

$116

Bisulfite, reduced representation DNA libraries (≥48 on robot)

$106

 

TELLSeq library construction, 1 library

 

$980

TELLSeq library construction, 2 libraries

$660

TELLSeq library construction, 3-4 libraries

$340

10X Single Cell Library Prep (any; kit provided) $591

10X CellPlex/Feature Barcoding add-on (per sample)

$307

10X Fixed RNA, 1 channel (up to 16 lib)

$14,010

10X Single Cell RNA Library (1 library)

$2,680

10x Single Cell RNA Library (2-7 samples, each)

$2,320

10x Single Cell RNA Library (8 samples submitted at once, each)

$2,090

10X Target Capture (baits provided; per pool)

$291

10X Single Nuclei ATAC Library, each

$2,670

10X Single Nuclei ATAC Library (2-7 samples, each)

$2,320

10X Single Nuclei ATAC Library (8 samples submitted at once, each)

$1,940

10X Single Nuclei ATAC+RNA Combined Library, each

$4,290

10X Single Nuclei ATAC+RNA Combined Library (2-7 samples, each)

$3,830

10X Single Nuclei ATAC+RNA Combined Library (8 samples at once, each)

$3,420

 

 

10x  Visium Spatial Transcriptomics Library (each, kit provided)

$150
   

Illumina NovaSeq X Plus 

(Now Available!)

 

10B flowcell= 1 billion read-pairs per lane

 
Nova X 10B paired-reads 300-cycle, per lane (1 to 3 lanes) $2,180
Nova X 10B paired-reads 300-cycle, per lane (4 to 7 lanes)   $2,060
Nova X 10B paired-reads 300-cycle, per lane (8 or more lanes) $1,840

10B flowcell= 1 billion single-reads per lane

 
Nova X 10B single-reads 100-cycle, per lane (1 to 3 lanes) $1,640
Nova X 10B single-reads 100-cycle, per lane (4 to 7 lanes) $1,570
Nova X 10B single-reads 100-cycle, per lane (8 or more lanes) $1,460
   

Illumina NovaSeq 6000

(we are an Illumina Certified Service Provider)

Price

SP flowcell    (per lane)

 
          Single-Reads, 100bp        (350+ million reads) $1,810
          Paired-Reads, 2x150bp    (750+ million reads) $2,510
          Paired-Reads, 2x250bp    (750+ million reads) $3,430

S1 flowcell    (per lane)

 
          Single-Reads, 100bp         (750+ million reads)

$3,590

          Paired-Reads, 2x100bp     (1.5+ billion reads) $4,610
          Paired-Reads, 2x150bp     (1.5+ billion reads) $4,930

S2 flowcell    (per lane)

 
          Single-Reads, 100bp      (1.5+ billion reads) $5,060

S4 flowcell    (per lane)

 
          Paired-Reads, 2x150bp   (5-6 billion reads) $5,970
 

 

Illumina MiSeq v3

Price

ISeq - titration of library pools (26nt + index read)

$579

ISeq 2x150nt PE

$842

MiSeq - titration of library pools (26nt + index read)

$374

MiSeq - 1x150nt SR v3

$1,400

MiSeq - 2x150nt PE NANO v2

$637

MiSeq - 2x150nt PE v2

$1,600

MiSeq - 2x250nt PE NANO v2

$690

MiSeq - 2x250nt PE v2

$1,750

MiSeq - 2x250nt PE v3

$2,160

MiSeq - 1x300nt SR v2

$1,600

MiSeq - 2x300nt PE v3

$2,220

MiSeq - 2x300 V3 Ready to Load $2,290

PacBio Sequel II

Price

Generation of full-length 16S amplicons, each

$29

Generation of full-length 16S amplicons, >48

$18

SMRTBell Library Construction from Amplicons

$320

QC of HMW DNA, Femto Pulse (1-10 samples)

$117

SMRTBell Library Construction, HiFi

$719

SMRTBell Library Construction, barcoded, 2

$423

SMRTBell Library Construction, barcoded, 3-11

$333

SMRTBell Library Construction, barcoded, 12+

$293

SMRTBell Library Construction, UltraLow

$835

SMRTBell Library Construction, UltraLow barcoded, 2-11

$685

SMRTBell Library Construction, UltraLow barcoded, 12+

$406

Iso-Seq Library Construction 

$485
Iso-Seq Library Construction (2+)

$371

PacBio Multiplexed Microbial Library (2+)

$269

PacBio Multiplexed Microbial Library (12+)

$202
PacBio Multiplexed Microbrial Library (24+)

$162

PacBio Multiplexed Microbrial Library (48+)

$135

Sequencing on SMRT 8M cell, Sequel II, 12h movie

$1,930
Corporate SMRT 8M cell, Sequel II, 12h movie

$2,410

Sequencing on SMRT 8M cell, Sequel II, 30h movie

$2,050
Corporate SMRT 8M cell, Sequel II, 30h movie

$2,530

PACBio 8M CCS demultiplexing fee

$224

Oxford-Nanopore GridION

Price

Construction of Oxford-Nanopore 1D libraries, standard

$418

Construction of Oxford-Nanopore 1D libraries, barcoded (2+)

$307

Construction of Oxford-Nanopore UltraLONG libraries, each

$415

Construction of Oxford-Nanopore Direct RNA libraries 

$376

Construction of Oxford-Nanopore Direct RNA libraries (2-6)

$332

Oxford-Nanopore GridION flowcell, each

$1,290

DNA/RNA Extraction

Price

DNA/RNA Extraction (each, 1-23)

$60

DNA/RNA Extraction (each, 24-47)

$51

DNA/RNA Extraction (each, 48-95)

$36

DNA/RNA Extraction (each, 96-191)

$29

DNA/RNA Extraction (each, 192+)

$23

HMW DNA Extraction (one)

$615

HMW DNA Extraction (two or more)

$352

Core Sequencing

Price

Sanger - High-Throughput Ready to Load (96-well Plate) - no clean up

$76

Sanger - High-Throughput Ready to Load (96-well Plate) -  with clean up

$210

Sanger - High-Throughput Sequencing (96-well plate)

$460

Sanger - Low-Throughput Sequencing (One Sample)

$6.00

Sanger - Low-Throughput Ready to Load (One Sample, includes clean-up)

$3.00

Fragment Analysis - High-Throughput (96-well Plate)

$101

Fragment Analysis - Low-Throughput (One Sample)

$3.90

Please visit the Sanger Sequencing and Fragment Analysis page for details regarding each service option. Please contact Core staff at 217-265-6814 or dna-seq@illinois.edu for any additional questions on Sanger Sequencing or Fragment Analysis.

This is an abridged list of our service prices and subject to change at any time.  For further information please contact Dr. Alvaro Hernandez, Director of DNA Services ( aghernan@illinois.edu ) at 217-244-3480 or Chris Wright, Assistant Director of DNA Services ( clwright@illinois.edu ) at 217-333-4372 to discuss ways the staff can be of assistance in achieving your project goals or to receive a quote for your project, submission forms, grant support or any other information needed.

External users are charged an additional 31.7% overhead fee, which is capped at 20% for those users from State of Illinois subsidized universities and government agencies. For further information on the contract, please contact Dr. Mark A. Mikel (217-244-0144; mmikel@illinois.edu ), Associate Director of the Roy J. Carver Biotechnology Center.

All scientific work performed by the Roy J. Carver Biotechnology Center (CBC)is required to be acknowledged in scholarly publications, posters, and presentations.  Proper recognition allows us to measure the impact of our work and supports our initiatives in obtaining sponsored funding. In addition, any CBC personnel who make a substantial intellectual or experimental contribution are deserving of further recognition as co-author.