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Pricing

Prior to project initiation, a meeting is required with CBC staff to discuss each project. If mutually agreed that the CBC can support the project, we will deliver a quote outlining the approach, timeline, and cost for the agreed service(s). As this is cutting-edge science, we cannot guarantee success. In all cases the user will be billed for cost and labor for CBC effort per our fee structure. After acceptance of the quote, the user will fill out and return the sample submission form provided with the quote, and also found on each CBC facility’s website. All samples are assumed to be safe, and no greater than BSL1, unless otherwise agreed upon in advance. The user must diligently and transparently follow all University of Illinois sample acquisition, movement, and safety protocols.

All pricing is updated, effective July 1st, 2021. Listed rates do not include the off-campus overhead (see bottom of page for overhead details).

Illumina NovaSeq 6000Ultra high-throughput sequencing of mRNA, genomic DNA, ChIP-DNA, small RNAs, bisulfite-treated DNA, single-cell transcriptomics, etc. For descriptions of library construction requirements and sequencing platform metrics, please visit our Sample Submission Page.

PacBio Sequel II: accurate long reads for de novo genome assembly and sequencing of long amplicons or cDNA.

Illumina Library Prep Services

Price

Sample or library quality control (QC)

 

Quality Check (QC) - one library (Qubit, Fragment Analyzer & qPCR)

$76

Quality Check  (QC) - 2-8 libraries (Qubit, Fragment Analyzer & qPCR)

$175

Sample Purification (eGel or SPRI bead. Does not include QC)

$86

Sample QC: Qubit & Fragment Analyzer (Minimum charge is for 8 samples)

$13

AATI Fragment Analyzer service (up to 96 samples or libraries)

$305

AATI Fragment Analyzer service (1-12 samples or libraries)

$67

AATI FemtoPulse (1-11 samples or libraries)

$115

HMW DNA Size Selection (40kb+, 1 or 2 samples)

$112
Covaris Annual User fee $200

Library Construction

 

BULK ATAC-Seq ActiveMotif library, <24 

$229

Shotgun genomic DNA library prep, each (1-11)

$138

Shotgun genomic DNA library prep, each (12-23)

$111

Shotgun genomic DNA library prep, each (24-47)

$90

Shotgun genomic DNA library prep, each (48-95)

$73

Shotgun genomic DNA library prep, each (96+)

$60

SkimSeq Libraries, each (48+)

$20

 

RNA-Seq library, PolyA selected RNA, each

$129

RNA-Seq library, PolyA selected RNA (≥24 on robot, full QC)

$89

RNA-Seq library, PolyA selected RNA (≥48 on robot, full QC)

$79

RNA-Seq library, PolyA selected RNA (≥96 on robot, full QC)

$69

 

RNA-Seq library, rRNA depleted, each

 

$200

RNA-Seq library from bacteria, rRNA depleted, each $156

RNA-Seq library, rRNA depleted (≥24 on robot, full QC)

$166

RNA-Seq library from bacteria, rRNA depleted (≥24 on robot, full QC) $128

RNA-Seq library, rRNA depleted (≥48 on robot, full QC)

$156

RNA-Seq library from bacteria, rRNA depleted (≥48 on robot, full QC) $118

RNA-Seq library, rRNA depleted (≥96 on robot, full QC)

   $146
RNA-Seq library from bacteria, rRNA depleted (≥96 on robot, full QC) $108

 

Small RNA libraries, each

 

$149

Small RNA libraries (≥24 on robot)

$117

Small RNA libraries (≥48 on robot)

   $107

Small RNA libraries (≥96 on robot)

   $97

 

Exome Capture libraries

$252

Exome Capture libraries (>48 on robot)

$181

 

Bisulfite, reduced representation DNA libraries, each

$171

Bisulfite, reduced representation DNA libraries (≥48 on robot)

$117

Bisulfite, genomic DNA libraries

$150

Bisulfite, genomic DNA libraries (≥48 on robot)

$93

 

TELLSeq library construction, 1Mb - <200Mb genome

 

$373

TELLSeq library construction, 200Mb - <1Gb genome

$454

TELLSeq library construction, 1Gb - 5Gb genome

$538

 

10X Single Cell RNA Low-Throughput Library (each)

 

$1070

10x CellPlex Sample Labeling (per sample)

$288

10X Single Cell RNA Library (1 library by itself, faster turnaround time)

$2330

10x Single Cell RNA Library (2-7 samples, each)

$2,030

10x Single Cell RNA Library (8 samples submitted at once, each)

$1,840

10X Target Capture (baits provided; per pool)

$309

10X Single Nuclei ATAC Library, each

$2180

10X Single Nuclei ATAC Library (2-7 samples, each)

$1880

10X Single Nuclei ATAC Library (8 samples submitted at once, each)

$1730

10X Single Nuclei ATAC+RNA Combined Library, each

$3480

10X Single Nuclei ATAC+RNA Combined Library (2-7 samples, each)

$3100

10X Single Nuclei ATAC+RNA Combined Library (8 samples at once, each)

$3000

 

IGB Tissue Optimization Labor estimate (per hr)

 

$68

IGB Tissue Section/Staining/cDNA prep labor estimate (per hr)

$68

10x  Visium Spatial Transcriptomics Library (each, kit provided)

$30

Illumina NovaSeq 6000

(we are an Illumina Certified Service Provider)

 

SP flowcell    (per lane)

 
          Single-Reads, 100bp        (350+ million reads) $1,730
          Paired-Reads, 2x150bp    (750+ million reads) $2,400
          Paired-Reads, 2x250bp    (750+ million reads) $3,260

S1 flowcell    (per lane)

 
          Single-Reads, 100bp         (750+ million reads)

$3,400

          Paired-Reads, 2x100bp     (1.5+ billion reads) $4,340
          Paired-Reads, 2x150bp     (1.5+ billion reads) $4,700

S2 flowcell    (per lane)

 
          Single-Reads, 100bp      (1.5+ billion reads) $4770

S4 flowcell    (per lane)

 
          Paired-Reads, 2x150bp   (5-6 billion reads) $5,930
 

 

Illumina MiSeq v3

Price

ISeq - titration of library pools (26nt + index read)

$440

ISeq 2x150nt PE

$746

MiSeq - titration of library pools (26nt + index read)

$299

MiSeq - 1x150nt SR v3

$1,400

MiSeq - 2x150nt PE NANO v2

$682

MiSeq - 2x150nt PE v2

$1,600

MiSeq - 2x250nt PE NANO v2

$746

MiSeq - 2x250nt PE v2

$1,740

MiSeq - 2x250nt PE v3

$2,110

MiSeq - 1x300nt SR v2

$1,600

MiSeq - 2x300nt PE v3

$2,200

PacBio Sequel II

 

Generation of full-length 16S amplicons, each

$30

Generation of full-length 16S amplicons, >48

$18

SMRTBell Library Construction from Amplicons

$421

QC of HMW DNA, Femto Pulse (1-10 samples)

$115

SMRTBell Library Construction, CLR

$732

SMRTBell Library Construction, HiFi

$732

SMRTBell Library Construction, barcoded, 2+

$431

SMRTBell Library Construction, barcoded, 12

$367

SMRTBell Library Construction, barcoded, 12+

$248

SMRTBell Library Construction, UltraLow

$874

SMRTBell Library Construction, UltraLow barcoded, 2+

$687

SMRTBell Library Construction, UltraLow barcoded, 12

$359

Iso-Seq Library Construction 

$524

PacBio Multiplexed Microbial Library (2+)

$233

PacBio Multiplexed Microbial Library (12+)

$192

Sequencing on SMRT 8M cell, Sequel II, 12h movie

$1,860

Sequencing on SMRT 8M cell, Sequel II, 30h movie

$1,980

PACBio 8M CCS demultiplexing fee

$106

Oxford-Nanopore GridION

 

Construction of Oxford-Nanopore 1D libraries, low input

$697

Construction of Oxford-Nanopore 1D libraries, low input (2-11)

$594

Construction of Oxford-Nanopore 1D libraries, low input (12+)

$466

Construction of Oxford-Nanopore 1D libraries, standard

$407

Construction of Oxford-Nanopore 1D libraries, barcoded (2-11)

$304

Construction of Oxford-Nanopore 1D libraries, barcoded (12+)

$127

Construction of Oxford-Nanopore Direct RNA libraries 

$307

Construction of Oxford-Nanopore Direct RNA libraries (2-6)

$256

Construction of Oxford-Nanopore Rapid DNA libraries 

$199

Construction of Oxford-Nanopore Rapid DNA libraries (12+)

$114

Oxford-Nanopore GridION flowcell, each

$960

DNA/RNA Extraction

 

DNA/RNA Extraction (each, 1-23)

$50

DNA/RNA Extraction (each, 24-47)

$35

DNA/RNA Extraction (each, 48-95)

$25

DNA/RNA Extraction (each, 96-191)

$20

DNA/RNA Extraction (each, 192+)

$15

HMW DNA Extraction (one)

$599

HMW DNA Extraction (two or more)

$399

Core Sequencing

Price

Sanger - High-Throughput Ready to Load (96-well Plate) - no clean up

$68

Sanger - High-Throughput Ready to Load (96-well Plate) -  with clean up

$158

Sanger - High-Throughput Sequencing (96-well plate)

$413

Sanger - Low-Throughput Sequencing (One Sample)

$5.00

Sanger - Low-Throughput Ready to Load (One Sample, includes clean-up)

$2.10

Fragment Analysis - High-Throughput (96-well Plate)

$89

Fragment Analysis - Low-Throughput (One Sample)

$2.60

Please visit the Sanger Sequencing and Fragment Analysis page for details regarding each service option. Please contact Core staff at 217-265-6814 or dna-seq@illinois.edu for any additional questions on Sanger Sequencing or Fragment Analysis.

This is an abridged list of our service prices and subject to change at any time.  For further information please contact Dr. Alvaro Hernandez, Director of DNA Services ( aghernan@illinois.edu ) at 217-244-3480 or Chris Wright, Assistant Director of DNA Services ( clwright@illinois.edu ) at 217-333-4372 to discuss ways the staff can be of assistance in achieving your project goals or to receive a quote for your project, submission forms, grant support or any other information needed.

Facility users not within the State of Illinois are subject to a 31.7% indirect cost rate. State of Illinois institutions are subject to a 31.7% indirect cost rate for Federally-funded projects and 10% indirect cost rate for non-Federally funded projects. For off-campus projects exceeding $10,000, a contract must be negotiated through University of Illinois Grants and Contracts office.  For further information on the contract, please contact Dr. Mark A. Mikel (217-244-0144; mmikel@illinois.edu ), Associate Director of the Roy J. Carver Biotechnology Center.

All scientific work performed by the Roy J. Carver Biotechnology Center (CBC)is required to be acknowledged in scholarly publications, posters, and presentations.  Proper recognition allows us to measure the impact of our work and supports our initiatives in obtaining sponsored funding. In addition, any CBC personnel who make a substantial intellectual or experimental contribution are deserving of further recognition as co-author.