Protocols
Optimize Your Flow Cytometry - Your Steps to Success
- DNA Content
- Cell Cycle Analysis
- Apoptosis Analysis
- Immunophenotyping
- Intracellular Antigen
- Cell Counting
- Cytometric Bead Array
- Functional Assays
- Cell Proliferation
- Surface Antigen Quantification
- Cell Preparation for Analysis and Sorting in CMtO
Need help designing your panel?
Here are some tools that can help you design your panel with minimal spillover and as per our instrument configurations:
1. Have a look at the fluorochrome reference chart to make sure you have fluorochromes that are compatible with our instruments configuration
2. Use the BD Biosciences spectrum viewer to look at the spectrum overlap between your fluorochromes
3. Have a look at the below image to help you assign your fluorochromes to the appropriate populations
You can find more information on how to select your multicolor fluorochromes here
4. Contact the CMtO staff if you have any questions at CMtO-Core@mx.uillinois.edu
BIOSAFETY RULES FOR CYTOMETRY AND MICROSCOPY TO OMICS USERS
All researchers and their staff must know the potential biohazards associated with their work. Cytometry and Microscopy to Omics is suitable as a BSL-1 (Biological Safety Level 1) laboratory in any facility, and as BSL-2 in 1230 DCL, 348 Burril Hall, 377 Morrill Hall, 264 RAL and in 231C ERML. BSL-3 work is never permitted. Notify Facility staff if you are planning work at a higher level than BSL-1.
All users must follow these guidelines:
-- NEVER bring food or drink in the laboratory.
-- NEVER bring any radioactive materials into any of our labs.
-- Self-service users are responsible for removing any trash from the bench areas, as well as cleaning up any spills.
-- Facility staff must be notified if the spill is hazardous. If you need help they will assist you in getting it cleaned up.
-- All sample-handling procedures must be done carefully to avoid generating aerosols.
-- The use of a lab coat and gloves are required for BSL-2 work. Change gloves immediately if they become contaminated.
-- Do not throw any laboratory materials into the regular trash. They may be discarded in our orange biohazard bags, but they must be decontaminated first. If you have biohazardous trash please dispose of it properly in your own lab. Samples containing hazardous chemicals (for example, propidium iodide) also must be taken back to your lab and held for pickup by the Division of Research Safety (http://www.drs.illinois.edu/).
-- Self-service users must follow the established protocols for decontamination/rinsing of the sample lines within the instruments and must properly dispose of all waste materials after every analysis session.
-- Any questions or comments please contact Cytometry and Microscopy to Omics at Cmto-core@mx.uillinois.edu, 217.300.5904
All work performed by the Roy J. Carver Biotechnology Center (CBC) should be acknowledged in scholarly publications, posters, and presentations. Proper recognition allows us to measure the impact of our work and supports our initiatives in obtaining sponsored funding. In addition, any CBC personnel who make a substantial intellectual or experimental contribution are deserving of further recognition as co-author.