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Illinois

Steps to Success in optimizing your samples for Cytometry and Microscopy to Omics

 

  1. Sample preparation - how to prepare a single cell suspension depending on your starting sample; viability tips for the healthiest sample.
  2. Staining considerations - basic steps and tips provided for cell surface, intracellular or nuclear staining; fixation and permeabilization tips
  3. Dead cell discrimination - explanation of importance, examples of fixable and unfixable dyes
  4. Doublet exclusion - avoid false positives 

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