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Illinois

Cell surface Staining Steps and Considerations 

Cell surface

Fc block

Fc block histo
Accessed from BIO-RAD

How to differentiate these populations?

Fc block

Present on NK cells, B cells, DCs, macrophages

  • bind Abs by the constant region 

    • - increased background staining

  • incubate with Fc block for 10-15 minutes

    • achieve clearer difference between positive population and isotype control