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Illinois

 BigFoot Invitrogen Thermofisher Spectral Cell Sorter

BigFoot Invitrogen Thermofisher Spectral Cell Sorter

 

Key System Specifications

  • Six-way sorter.
  • Sort rates exceeding 70,000 events per second, and analysis rates greater than 100,000 events per second.
  • Multi-well sorting into 96-well, 384-well plates. Straight-down sorting into 1536-well plates.
  • Excitation lasers at 349 nm, 405 nm, 488 nm, 561 nm, 640 nm.
  • Detection parameters up to 55 fluorescence with 5 scatters.
  • 70, 100, 120, and 150 µm ceramic nozzle tips with adjustable pressure.
  • Capable of output holders with 1.5, 5-, 15-, and 50-mL tubes

Bigfoot Laser Diagram

 

CMtO Bigfoot Cell Sorter Configuration:

Figure: 349nm BankFigure: 405nm BankFigure: 640/488nm BankFigure: 561nm Bank

 

Please Click for More Information:
BigFoot QuickStart Guide.pdf
BigFoot Technical Specifications.pdf
BigFoot User Brochure.pdf

Fluorophore and reagent selection guide 

Thermofisher spectra viewer

Designing a panel based on fluorochrome performance: Spillover vs Resolution

Manufacturer: ThermoFisher Scientific
Equipment Model: Bigfoot
Location: Main Facility at 231 Edward R. Madigan Laboratory
Contact Person: Marcin Wozniak: cmto-core@mx.uillinois.edu