The Cytometric Bead Array System
Picture obtained from BD Cytometric Bead Array – Multiplexed Bead-Based Immunoassays
If you are looking for a good alternative to enzyme-linked immunosorbent assays to efficiently and simultaneously measure different panels of analytes (chemokines, cytokines, phosphoproteins, growth factors, human immunoglobulins etc.) in a single microplate well, then multiplex bead assay is your choice.
Our Main Facility is equipped with a Bio-Rad Luminex Cytometric Bead Analyzer, which can perform up to 40 assays (each bead is infused with fluorescent dyes in different ratios allowing for emission of different amounts of photons depending upon the amount of binding) in a single well, increasing assay flexibility as it can be used to perform immunoassays, nucleic acid research, enzymatic research, and receptor-ligand studies.
Picture obtained from BIO-RAD – Multiplex Immunoassays
Multiple kits are available on the market, preconfigured or configurable kits (build your own multiplexes).
BD CBA Flex Sets - BD Biosciences
BD CBA Kits - BD Biosciences
Immunoassays and Multiplex kits - EMD Millipore
FlowCytomix Multiplex kits - affymetrix eBioscience
Human cytokine Multiplex kits - Life Technologies
Mouse cytokine Multiplex kits - Life Technologies
Bio-Plex Suspension Array System - BIO-RAD Luminex Assays and Luminex High Performance Assays - simultaneous detection and quantification of multiple target analytes in qualified complex sample types. Designed to provide accurate, reproducible results for every target analyte.
A paper to read if you want to know more:
Bead-Based Multianalyte Flow Immunoassays - The Cytometric Bead Array System
Scheduling
Please contact us to schedule an appointment or to discuss a future project: kjans01s@illinois.edu, (217) 300.5904.