Steps to Success in optimizing your samples for Cytometry and Microscopy to Omics
- Sample preparation - how to prepare a single cell suspension depending on your starting sample; viability tips for the healthiest sample.
- Staining considerations - basic steps and tips provided for cell surface, intracellular or nuclear staining; fixation and permeabilization tips
- Dead cell discrimination - explanation of importance, examples of fixable and unfixable dyes
- Doublet exclusion - avoid false positives