Doublet Exclusion
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•Exclusion is necessary to avoid false positives •Scatter plot FSC-W vs FCS-A or FSC-A vs FSC-H •Doublets will have higher width than singlets •Very important in cell cycle as doublets may show as different ploidy
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•Exclusion is necessary to avoid false positives •Scatter plot FSC-W vs FCS-A or FSC-A vs FSC-H •Doublets will have higher width than singlets •Very important in cell cycle as doublets may show as different ploidy
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