10X FFPE CytAssist Visium
- Sample workflow of 10x Visium involving CBC-CMtO, DNA Services, Proteomics and Metabolomics
- 10X FFPE CytAssist Visium (website)
- CytAssist Short Brochure (pdf)
- 10x CytAssist and how it works video?
10x Visium CytAssist FFPE Service
Key System Specifications
- Whole tissue section profiling, eliminating need for sections of interest
- 1-10 cell resolution, depending on type of tissue
- Accommodating for diverse organ samples among various species
- Human, mouse, rat, and others
- Two capture area sizes for compatibility with larger tissue samples
- 6.5 x 6.5 mm and 11 x 11 mm
- RNA-templated litigation (RTL) probe allows for high specificity and sensitivity for entire transcriptome of human and mouse
- Compatible with histological and immunofluorescent sections
High Resolution Histology section obtained image from LSM 980 and the corresponding CytAssist image acquired in the CytAssist system at the time of RNA extraction, to be overlaid for spatial transcriptomic analysis.
Section location and alignment requirements for CytAssist FFPE
Please Click for More Information:
CytAssist FFPE Workflow (pdf)
NOW OFFERING - 10X VISIUM HD
Visium HD empowers a new era of spatial discovery, enhancing proven whole transcriptome spatial analysis with single cell-scale resolution, enabling continuous tissue coverage, and delivering best-in-class data with innovation probe-based chemistry and a Visium CytAssist-enabled workflow.
What type of samples are compatible with the visium HD?
Currently, Visium HD is compatible with human and mouse formalin-fixed paraffin-embedded (FFPE) tissue sections containing mRNA. This includes archived FFPE blocks and pre-sectioned tissue on glass slides.
Click any of the links below for more information:
10x genomics Visium Cytassist HD
Visium HD in FFPE human breast cancer at single cell scale.pdf
Grant application resources for visium products.pdf
Visium_HD_Flyer_Launch_Letter_Digital.pdf
Can we do plant samples on Visium?
Manufacturer: 10X Genomics
Equipment Model: 10x Visium CytAssist
Location: Main Facility at 231 Edward R. Madigan Laboratory
Contact Person: Mayandi Sivaguru, sivaguru@illinois.edu