Pricing
Prior to project initiation, a meeting is required with CBC staff to discuss each project. If mutually agreed that the CBC can support the project, we will deliver a quote outlining the approach, timeline, and cost for the agreed service(s). As this is cutting-edge science, we cannot guarantee success. In all cases the user will be billed for cost and labor for CBC effort per our fee structure. After acceptance of the quote, the user will fill out and return the sample submission form provided with the quote, and also found on each CBC facility’s website. All samples are assumed to be safe, and no greater than BSL1, unless otherwise agreed upon in advance. The user must diligently and transparently follow all University of Illinois sample acquisition, movement, and safety protocols.
All pricing is updated, effective July 1st, 2023. Listed rates do not include the off-campus overhead (see bottom of page for overhead details).
Illumina NovaSeq X Plus and 6000: Ultra high-throughput sequencing of mRNA, genomic DNA, ChIP-DNA, small RNAs, bisulfite-treated DNA, single-cell transcriptomics, etc. For descriptions of library construction requirements and sequencing platform metrics, please visit our Sample Submission Page.
PacBio REVIO: accurate long reads for de novo genome assembly and sequencing of long amplicons or cDNA.
Illumina Library Prep Services |
Price |
|
Sample or library quality control (QC) |
|
| Hourly rate, cell test counts, other (per hour) | $90 |
|
Quality Check (QC) - one library (Qubit, Fragment Analyzer & qPCR) |
$109 |
|
Quality Check (QC) - 2-8 libraries (Qubit, Fragment Analyzer & qPCR) |
$258 |
|
Sample Purification (eGel or SPRI bead. Does not include QC) |
$161 |
|
Sample QC: Qubit & Fragment Analyzer (Minimum charge is for 8 samples) |
$14 |
|
AATI Fragment Analyzer service (up to 96 samples or libraries) |
$417 |
|
AATI Fragment Analyzer service (1-12 samples or libraries) |
$97 |
|
AATI FemtoPulse (1-11 samples or libraries) |
$144 |
|
HMW DNA Size Selection (40kb+, 1 or 2 samples) |
$247 |
| Covaris Annual User fee | $200 |
|
Library Construction |
|
|
BULK ATAC-Seq ActiveMotif library, <24 |
$191 |
|
Shotgun genomic PCR-free prep, each |
$172 |
|
Shotgun genomic DNA library prep, each (1-11) |
$147 |
|
Shotgun genomic DNA library prep, each (12-23) |
$106 |
|
Shotgun genomic DNA library prep, each (24-47) |
$87 |
|
Shotgun genomic DNA library prep, each (48-95) |
$72 |
|
Shotgun genomic DNA library prep, each (96+) |
$61 |
|
SkimSeq Libraries, each (48+) |
$23 |
| SkimSeq Libraries, each (48+; plus QC) | $27 |
|
RNA-Seq library, PolyA selected RNA, each |
$119 |
|
RNA-Seq library, PolyA selected RNA (≥12 on robot, full QC) |
$101 |
|
RNA-Seq library, PolyA selected RNA (≥24 on robot, full QC) |
$86 |
|
RNA-Seq library, PolyA selected RNA (≥48 on robot, full QC) |
$79 |
|
RNA-Seq library, PolyA selected RNA (≥96 on robot, full QC) |
$71 |
|
RNA-Seq library, rRNA depleted, each |
$171 |
|
RNA-Seq library, rRNA depleted (≥12 on robot, full QC) |
$152 |
|
RNA-Seq library, rRNA depleted (≥24 on robot, full QC) |
$125 |
|
RNA-Seq library, rRNA depleted (≥48 on robot, full QC) |
$118 |
|
RNA-Seq library, rRNA depleted (≥96 on robot, full QC) |
$110 |
|
Small RNA libraries, each |
$163 |
|
Small RNA libraries (≥24 on robot) |
$131 |
| Small RNA libraries (≥48 on robot) | $123 |
| Small RNA libraries (≥96 on robot) | $116 |
|
Bacterial Small RNA libraries (≥24 on robot) |
$170 |
|
Bacterial Small RNA libraries (≥48 on robot) |
$163 |
|
Bacterial Small RNA libraries (≥96 on robot) |
$155 |
|
Exome Capture libraries |
$189 |
|
Exome Capture libraries (>48 on robot) |
$157 |
|
Bisulfite, reduced representation DNA libraries, each |
$140 |
|
Bisulfite, reduced representation DNA libraries (≥24 on robot) |
$116 |
|
Bisulfite, reduced representation DNA libraries (≥48 on robot) |
$106 |
|
TELLSeq library construction, 1 library |
$980 |
|
TELLSeq library construction, 2 libraries |
$660 |
|
TELLSeq library construction, 3-4 libraries |
$340 |
| 10X Single Cell Library Prep (any; kit provided) | $591 |
|
10X CellPlex/Feature Barcoding add-on (per sample) |
$307 |
|
10X Fixed RNA, 1 channel (up to 16 lib) |
$14,010 |
|
10X Single Cell RNA Library (1 library) |
$2,680 |
|
10x Single Cell RNA Library (2-7 samples, each) |
$2,320 |
|
10x Single Cell RNA Library (8 samples submitted at once, each) |
$2,090 |
|
10X Target Capture (baits provided; per pool) |
$291 |
|
10X Single Nuclei ATAC Library, each |
$2,670 |
|
10X Single Nuclei ATAC Library (2-7 samples, each) |
$2,320 |
|
10X Single Nuclei ATAC Library (8 samples submitted at once, each) |
$1,940 |
|
10X Single Nuclei ATAC+RNA Combined Library, each |
$4,290 |
|
10X Single Nuclei ATAC+RNA Combined Library (2-7 samples, each) |
$3,830 |
|
10X Single Nuclei ATAC+RNA Combined Library (8 samples at once, each) |
$3,420 |
|
|
|
|
10x Visium Spatial Transcriptomics Library (each, kit provided) |
$150 |
Illumina NovaSeq X Plus(Now Available!) |
|
10B flowcell= 1 billion read-pairs per lane |
|
| Nova X 10B paired-reads 300-cycle, per lane (1 to 3 lanes) | $2,180 |
| Nova X 10B paired-reads 300-cycle, per lane (4 to 7 lanes) | $2,060 |
| Nova X 10B paired-reads 300-cycle, per lane (8 or more lanes) | $1,840 |
10B flowcell= 1 billion single-reads per lane |
|
| Nova X 10B single-reads 100-cycle, per lane (1 to 3 lanes) | $1,640 |
| Nova X 10B single-reads 100-cycle, per lane (4 to 7 lanes) | $1,570 |
| Nova X 10B single-reads 100-cycle, per lane (8 or more lanes) | $1,460 |
25B flowcell= 3.2 billion read-pairs per lane |
|
| Nova X 25B paired-reads 300-cycle, per lane (1-7 lanes) | $3,240 |
| Nova X 25B paired-reads 300-cycle, per lane | $3,080 |
Illumina NovaSeq 6000(we are an Illumina Certified Service Provider) |
Price |
|
SP flowcell (per lane) |
|
| Single-Reads, 100bp (350+ million reads) | $1,810 |
| Paired-Reads, 2x150bp (750+ million reads) | $2,510 |
| Paired-Reads, 2x250bp (750+ million reads) | $3,430 |
|
S1 flowcell (per lane) |
|
| Single-Reads, 100bp (750+ million reads) |
$3,590 |
| Paired-Reads, 2x100bp (1.5+ billion reads) | $4,610 |
| Paired-Reads, 2x150bp (1.5+ billion reads) | $4,930 |
|
S2 flowcell (per lane) |
|
| Single-Reads, 100bp (1.5+ billion reads) | $5,060 |
|
S4 flowcell (per lane) |
|
| Paired-Reads, 2x150bp (5-6 billion reads) | $5,970 |
|
|
Illumina MiSeq v3 |
Price |
|
ISeq - titration of library pools (26nt + index read) |
$579 |
|
ISeq 2x150nt PE |
$842 |
|
MiSeq - titration of library pools (26nt + index read) |
$374 |
|
MiSeq - 1x150nt SR v3 |
$1,400 |
|
MiSeq - 2x150nt PE NANO v2 |
$637 |
|
MiSeq - 2x150nt PE v2 |
$1,600 |
|
MiSeq - 2x250nt PE NANO v2 |
$690 |
|
MiSeq - 2x250nt PE v2 |
$1,750 |
|
MiSeq - 2x250nt PE v3 |
$2,160 |
|
MiSeq - 1x300nt SR v2 |
$1,600 |
|
MiSeq - 2x300nt PE v3 |
$2,220 |
| MiSeq - 2x300 V3 Ready to Load | $2,290 |
PacBio REVIO |
Price |
|
Generation of full-length 16S amplicons, each |
$29 |
|
Generation of full-length 16S amplicons, >48 |
$18 |
|
SMRTBell Library Construction from Amplicons |
$320 |
|
QC of HMW DNA, Femto Pulse (1-10 samples) |
$144 |
|
SMRTBell Library Construction, HiFi |
$719 |
|
SMRTBell Library Construction, barcoded, 2 |
$423 |
|
SMRTBell Library Construction, barcoded, 3-11 |
$333 |
|
SMRTBell Library Construction, barcoded, 12+ |
$293 |
|
SMRTBell Library Construction, UltraLow |
$835 |
|
SMRTBell Library Construction, UltraLow barcoded, 2-11 |
$685 |
|
SMRTBell Library Construction, UltraLow barcoded, 12+ |
$406 |
|
Iso-Seq Library Construction |
$485 |
| Iso-Seq Library Construction (2+) |
$371 |
|
PacBio Multiplexed Microbial Library (2+) |
$269 |
|
PacBio Multiplexed Microbial Library (12+) |
$202 |
| PacBio Multiplexed Microbrial Library (24+) |
$162 |
| PacBio Multiplexed Microbrial Library (48+) |
$135 |
| PacBio Multiplexed Microbrial Library (96+) |
$90 |
| Kinnex 16S Library Construction |
$989 |
| Kinnex 16S Library Construction, 2+ |
$812 |
| Kinnex RNA Library Construction |
$1,140 |
| Kinnex RNA Library Construction, 2-4, each |
$670 |
| Kinnex RNA Library Construction, 5-11, each |
$656 |
| Kinnex RNA Library Construction, 48+, each |
$630 |
|
Sequencing on SMRT REVIO cell, 12h movie |
$1,930 |
| Corporate SMRT REVIO cell, 12h movie |
$2,410 |
|
Sequencing on SMRT REVIO cell, 30h movie |
$2,050 |
| Corporate SMRT REVIO cell, 30h movie |
$2,590 |
|
PACBio 8M CCS demultiplexing fee |
$224 |
Oxford-Nanopore GridION |
Price |
|
Construction of Oxford-Nanopore 1D libraries, standard |
$418 |
|
Construction of Oxford-Nanopore 1D libraries, barcoded (2+) |
$307 |
|
Construction of Oxford-Nanopore UltraLONG libraries, each |
$415 |
|
Construction of Oxford-Nanopore Direct RNA libraries |
$376 |
|
Construction of Oxford-Nanopore Direct RNA libraries (2-6) |
$332 |
|
Oxford-Nanopore GridION flowcell, each |
$1,290 |
DNA/RNA Extraction |
Price |
|
DNA/RNA Extraction (each, 1-23) |
$60 |
|
DNA/RNA Extraction (each, 24-47) |
$51 |
|
DNA/RNA Extraction (each, 48-95) |
$36 |
|
DNA/RNA Extraction (each, 96-191) |
$29 |
|
DNA/RNA Extraction (each, 192+) |
$23 |
|
HMW DNA Extraction (one) |
$615 |
|
HMW DNA Extraction (two or more) |
$352 |
Core Sequencing |
Price |
|
Sanger - High-Throughput Ready to Load (96-well Plate) - no clean up |
$76 |
|
Sanger - High-Throughput Ready to Load (96-well Plate) - with clean up |
$210 |
|
Sanger - High-Throughput Sequencing (96-well plate) |
$460 |
|
Sanger - Low-Throughput Sequencing (One Sample) |
$6.00 |
|
Sanger - Low-Throughput Ready to Load (One Sample, includes clean-up) |
$3.00 |
|
Fragment Analysis - High-Throughput (96-well Plate) |
$101 |
|
Fragment Analysis - Low-Throughput (One Sample) |
$3.90 |
Please visit the Sanger Sequencing and Fragment Analysis page for details regarding each service option. Please contact Core staff at 217-265-6814 or dna-seq@illinois.edu for any additional questions on Sanger Sequencing or Fragment Analysis.
This is an abridged list of our service prices and subject to change at any time. For further information please contact Dr. Alvaro Hernandez, Director of DNA Services ( aghernan@illinois.edu ) at 217-244-3480 or Chris Wright, Assistant Director of DNA Services ( clwright@illinois.edu ) at 217-333-4372 to discuss ways the staff can be of assistance in achieving your project goals or to receive a quote for your project, submission forms, grant support or any other information needed.
External users are charged an additional 31.7% overhead fee, which is capped at 20% for those users from State of Illinois subsidized universities and government agencies. For further information on the contract, please contact Dr. Marcello Rubessa (rubessa@illinois.edu), Associate Director of the Roy J. Carver Biotechnology Center.
All scientific work performed by the Roy J. Carver Biotechnology Center (CBC)is required to be acknowledged in scholarly publications, posters, and presentations. Proper recognition allows us to measure the impact of our work and supports our initiatives in obtaining sponsored funding. In addition, any CBC personnel who make a substantial intellectual or experimental contribution are deserving of further recognition as co-author.