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University of Illinois

Instrumentation

Our facility utilizes 9 mass spectrometers for conducting untargeted and targeted metabolomics analysis. This includes 6 LC-MS instruments such as 3 triple quadrupole MS for targeted metabolomics analysis and 3 high-resolution MS for untargeted metabolomics analysis. We also have 3 quadrupole GC-MS instruments for targeted metabolomics analysis that includes largescale metabolite profiling. We no longer have a multi-label microplate reader. This can now be used 24/7 at the Materials Research Lab on-campus.


PacBio

Agilent LC-MS - 6560 Ion Mobility Q-TOF Mass Spectrometer

Our lab purchased an Agilent 6560C ion mobility Q-TOF LC/MS system* that enables optimal separation and selectivity by combining chromatography, ion mobility, and mass spectrometry that enable metabolite identification through collection of retention time, collision cross section (CCS), m/z, and MS2 data, respectively. This includes separation of in-class isomers via high-resolution demultiplexing (HRdm). It has the following features:

  • In-spectrum Dynamic Range: 5 orders of magnitude
  • MS and MS/MS Mass Accuracy: <1 and <2 ppm RMS, respectively
  • Ion Mobility Resolution: 200 FWHM
  • TOF Mass Resolution: >42,000 FWHM
  • TOF mass range: m/z 20 - 20,000
  • Connected with Agilent 1290 Infinity II UHPLC

For: Untargeted analysis (primarily Lipidomics)

*Installation is in progress and to be completed in February 24'


6546

Agilent LC-MS - 6546 Q-TOF Mass Spectrometer

The Agilent 6546 Q-TOF LC/MS system offers simultaneous high sensitivity, wide dynamic range, high resolution, isotope fidelity, and accurate mass measurements. It has the following features:

  • In-spectrum Dynamic Range: 5 orders of magnitude
  • MS and MS/MS Mass Accuracy <0.8 and <2 ppm RMS, respectively
  • TOF Mass Resolution: >60,000 FWHM
  • TOF mass range: m/z 20 - 10,000
  • Connected with Agilent 1290 Infinity II UHPLC

For: Untargeted analysis


PacBio

Thermo Scientific LC-MS - Q Exactive Mass Spectrometer

This Hybrid Quadrupole-Orbitrap system combines quadruple precursor ion selection with high-resolution,
accurate-mass (HRAM) Orbitrap detection to help identify and confirm more metabolites. It has the following
features:

  • Better than 3ppm mass accuracy in full-scan with external calibration
  • Resolving power: 140,000 FWHM
  • Extended mass range: m/z 50 - 6,000
  • Connected with Ultimate 3000 UHPLC along with diode array detector (DAD)

For: Untargeted analysis


Sciex LC-MS6500 Triple Quadrupole MS

Sciex LC-MS - 6500+ Triple Quadrupole Mass Spectrometer

A benchtop triple Quadrupole mass spectrometer designed for LC/MS/MS analysis. Available IonDrive™ Turbo V Source includes ESI, and APCI. It has the following features:

  • Scan modes include: full scan and selected ion scan for both Q1 and Q3, Product Ion Scan, Precursor Ion Scan, Neutral Loss Scan, Multiple Reaction Monitoring (MRM)
  • Mass range of m/z 5 ~ m/z 2,000
  • Up to 6 orders of linear dynamic range
  • Increased throughput with polarity switching speeds of 5 msec
  • Connected with Agilent 1290 Infinity II UHPLC

For: Targeted analysis


Sciex LC-MS5500 QTrap MS

Sciex LC-MS - 5500 QTrap Mass Spectrometer

A benchtop hybrid triple Quadrupole-linear accelerator trap mass spectrometer designed for LC/MS/MS analysis. Available Turbo V™ Source includes ESI, and APCI. It has the following features:

  • Scan modes include: full scan and selected ion scan for both Q1 and Q3, Product Ion Scan, Precursor Ion Scan, Neutral Loss Scan, Multiple Reaction Monitoring (MRM), Enhanced MS Scan, Enhanced Product Ion Scan, Enhanced Resolution Scan, and MS3 scan
  • Mass range of m/z 5 ~ m/z 1,250
  • Connected with Agilent 1290 Infinity II UHPLC

For: Targeted analysis


Altis Triple Quadrupole Mass spectrometer

Thermo LC-MS - Altis Triple Quadrupole Mass Spectrometer

The TSQ Altis MS is designed for stable, reliable and consistent performance. It has the following features:

  • Mass range: m/z 5 ~ m/z 2,000
  • Equipped with ESI source
  • Operated with Tracefinder software
  • Connected with Thermo Vanquish UHPLC system
  • Ultrafast selected reaction monitoring (SRM) increases the amount of molecular quantifications in less time

For: Targeted analysis


Agilent GC-MS Utmost quantitative analysis

Agilent GC-MS - 5977A Mass Spectrometer

A benchtop mass spectrometer designed for small metabolite analysis. It has the following features:

  • Equipped with EI and CI sources.
  • 7890B GC with 5977A Extractor XL MS
  • With commercial Wiley and NIST libraries
  • Mass range of m/z 2 ~ m/z 800
  • Operated with MassHunter Software

For: Targeted analysis and profiling


Agilent GC-MS - 5975C Mass Spectrometer

A benchtop mass spectrometer designed for small metabolite analysis. It has the following features:

  • Equipped with EI and CI sources.
  • 7890A GC with 5975C MS
  • With commercial Wiley and NIST libraries
  • Mass range of m/z 2 ~ m/z 800

For: Targeted analysis and profiling


Agilent GC-MS Targeted metabolite Profiling

Agilent GC-MS - 5973 Mass Spectrometer

A benchtop mass spectrometer designed for small metabolite analysis. It has the following features:

  • Equipped with EI and CI sources
  • 6890N GC with 5973 MS
  • Mass range of m/z 2 ~ m/z 800

For: Targeted analysis


Note:

We no longer manage a SpectraMax M2 Multilabel Microplate Reader. Those interested in its use can contact the MRL facility on-campus.