Microbial Aromatic Amino Acid Derivatives
Targeted method quantifying 24 microbial derivatives of the aromatic amino acids' tryptophan, phenylalanine, and tyrosine using LC-MS/MS with MRM. Quantitation is performed using calibrations of up to 10 concentration levels and 5 labeled internal standards.
| Metabolite Name | Amino Acid Precursors | PubChem CID | InChIKey |
| Kynurenine | Tryptophan | 161166 | YGPSJZOEDVAXAB-QMMMGPOBSA-N |
| Kynurenic acid | Tryptophan | 24729582 | HCZHHEIFKROPDY-UHFFFAOYSA-N |
| Quinolinic acid | Tryptophan | 1066 | GJAWHXHKYYXBSV-UHFFFAOYSA-N |
| Tryptamine | Tryptophan | 1150 | APJYDQYYACXCRM-UHFFFAOYSA-N |
| Serotonin | Tryptophan | 5202 | QZAYGJVTTNCVMB-UHFFFAOYSA-N |
| Indole | Tryptophan | 798 | SIKJAQJRHWYJAI-UHFFFAOYSA-N |
| Indole-3-lactic acid | Tryptophan | 92904 | XGILAAMKEQUXLS-UHFFFAOYSA-N |
| Indole-3-acetic acid | Tryptophan | 802 | SEOVTRFCIGRIMH-UHFFFAOYSA-N |
| Indole-3-acrylic acid | Tryptophan | 5375048 | PLVPPLCLBIEYEA-AATRIKPKSA-N |
| Indole-3-propionic acid | Tryptophan | 3744 | GOLXRNDWAUTYKT-UHFFFAOYSA-N |
| Indole-3-pyruvic acid | Tryptophan | 5460337 | RSTKLPZEZYGQPY-UHFFFAOYSA-N |
| Indole-carboxyaldehyde | Tryptophan | 10256 | OLNJUISKUQQNIM-UHFFFAOYSA-N |
| 3-Phenyllactic acid | Phenylalanine | 17750388 | VOXXWSYKYCBWHO-UHFFFAOYSA-N |
| 3-Phenylpropionic acid | Phenylalanine | 107 | XMIIGOLPHOKFCH-UHFFFAOYSA-N |
| Phenylacetic acid | Phenylalanine | 999 | WLJVXDMOQOGPHL-UHFFFAOYSA-N |
| Phenylacetyl-L-glutamine | Phenylalanine | 92258 | JFLIEFSWGNOPJJ-JTQLQIEISA-N |
| Phenylacetyl-L-glycine | Phenylalanine | 68144 | UTYVDVLMYQPLQB-UHFFFAOYSA-N |
| Phenylpyruvic acid | Phenylalanine | 997 | BTNMPGBKDVTSJY-UHFFFAOYSA-N |
| t-Cinnamic acid | Phenylalanine | 444539 | WBYWAXJHAXSJNI-VOTSOKGWSA-N |
| p-Coumaric acid | Phenylalanine/Tyrosine | 1549106 | NGSWKAQJJWESNS-ZZXKWVIFSA-N |
| 4-Hydroxyphenylacetic acid | Phenylalanine/Tyrosine | 127 | XQXPVVBIMDBYFF-UHFFFAOYSA-N |
| 4-Hydroxyphenylpyruvic acid | Phenylalanine/Tyrosine | 979 | KKADPXVIOXHVKN-UHFFFAOYSA-N |
| 3-(4-hydroxyphenyl)lactic acid | Phenylalanine/Tyrosine | 9920545 | JVGVDSSUAVXRDY-UHFFFAOYSA-N |
| 3-(4-Hydroxyphenyl)propionic acid | Phenylalanine/Tyrosine | 10394 | NMHMNPHRMNGLLB-UHFFFAOYSA-N |
Note: The method is also able to detect precursors' tryptophan, phenylalanine, and tyrosine. However, due to their high concentrations, these 3 metabolites cannot be quantified within the same sample run as the Microbial Aromatic Amines panel. At extra cost, a separate sample run can be performed.