Assay: Amino Acids
Targeted method quantifying amino acids using LC-MS/MS with MRM. Quantitation is performed using calibrations of up to 10 concentration levels and 9 labeled internal standards. Standard reference material (SRM) 1950 containing certified and reference values for amino acids is also used.
| Metabolite Name | PubChem CID | InChIKey |
| Alanine | 5950 | QNAYBMKLOCPYGJ-REOHCLBHSA-N |
| Arginine | 66250 | KWTQSFXGGICVPE-WCCKRBBISA-N |
| Asparagine | 6267 | DCXYFEDJOCDNAF-REOHCLBHSA-N |
| Aspartic acid | 5960 | CKLJMWTZIZZHCS-REOHCLBHSA-N |
| Cysteine | 5862 | XUJNEKJLAYXESH-REOHCLBHSA-N |
| Cystin | 67678 | LEVWYRKDKASIDU-IMJSIDKUSA-N |
| Glutamine | 5961 | ZDXPYRJPNDTMRX-VKHMYHEASA-N |
| Glutamic acid | 33032 | WHUUTDBJXJRKMK-VKHMYHEASA-N |
| Glycine | 750 | DHMQDGOQFOQNFH-UHFFFAOYSA-N |
| L-Histidine | 165377 | CMXXUDSWGMGYLZ-XRIGFGBMSA-N |
| Isoleucine | 6306 | AGPKZVBTJJNPAG-WHFBIAKZSA-N |
| Leucine | 6106 | ROHFNLRQFUQHCH-YFKPBYRVSA-N |
| L-Lysine | 69568 | BVHLGVCQOALMSV-JEDNCBNOSA-N |
| Methionine | 6137 | FFEARJCKVFRZRR-BYPYZUCNSA-N |
| Phenylalanine | 6140 | COLNVLDHVKWLRT-QMMMGPOBSA-N |
| Proline | 145742 | ONIBWKKTOPOVIA-BYPYZUCNSA-N |
| Serine | 5951 | MTCFGRXMJLQNBG-REOHCLBHSA-N |
| Threonine | 6288 | AYFVYJQAPQTCCC-GBXIJSLDSA-N |
| Tryptophan | 6305 | QIVBCDIJIAJPQS-VIFPVBQESA-N |
| Tyrosine | 6057 | OUYCCCASQSFEME-QMMMGPOBSA-N |
| Valine | 6287 | KZSNJWFQEVHDMF-BYPYZUCNSA-N |