Assay: Bile Acids
Targeted method identifying up to 28 bile acids using LC-MS/MS with MRM. Quantitation is performed using up to 10 calibration standard levels and 8 isotopically labeled surrogate internal standards. Bile acids consist of primary and secondary conjugates and unconjugates, including those unique to specific species.
| Metabolite Name | PubChem CID | InChIKey |
| Cholic acid | 221493 | BHQCQFFYRZLCQQ-OELDTZBJSA-N |
| Chenodeoxycholic acid | 10133 | RUDATBOHQWOJDD-BSWAIDMHSA-N |
| Deoxycholic acid | 222528 | KXGVEGMKQFWNSR-LLQZFEROSA-N |
| Glycocholic acid | 10140 | RFDAIACWWDREDC-FRVQLJSFSA-N |
| Glychochenodeoxycholic acid | 12544 | DPKVEQOULFKWCR-KZRRTRIISA-M |
| Glycoursodeoxycholic acid | 12310288 | GHCZAUBVMUEKKP-XROMFQGDSA-N |
| Taurocholic acid | 6675 | WBWWGRHZICKQGZ-HZAMXZRMSA-N |
| Glycodeoxycholic acid | 3035026 | WVULKSPCQVQLCU-BUXLTGKBSA-N |
| Glycohyodeoxycholic acid | 114611 | SPOIYSFQOFYOFZ-BRDORRHWSA-N |
| Glycolithocholic acid | 115245 | XBSQTYHEGZTYJE-OETIFKLTSA-N |
| Taurohyocholic acid | NYXROOLWUZIWRB-YOAOXILXSA-N | |
| Glycohyocholic acid | 71361462 | ZQYUKJFJPJDMMR-ZDWCHQGWSA-N |
| Glycohyodeoxycholic acid | 114611 | SPOIYSFQOFYOFZ-BRDORRHWSA-N |
| Hyocholic acid | 92805 | DKPMWHFRUGMUKF-KWXDGCAGSA-N |
| Hyodeoxycholic acid | 5283820 | DGABKXLVXPYZII-SIBKNCMHSA-N |
| Lithocholic acid | 9903 | SMEROWZSTRWXGI-HVATVPOCSA-N |
| Taurochenodeoxycholic acid | 23664008 | IYPNVUSIMGAJFC-HLEJRKHJSA-M |
| Taurodeoxycholic acid | 23702150 | OLPIZAYYAVQETM-GGPRKOIFSA-M |
| Taurolithocholic acid | 23662757 | YAERYJYXPRIDTO-HRHHVWJRSA-M |
| Tauroursodeoxycholic acid | 9848818 | BHTRKEVKTKCXOH-LBSADWJPSA-N |
| Ursodeoxycholic acid | 23707110 | WDFRNBJHDMUMBL-FUXQPCDDSA-M |
| α-Muricholic acid | 5283852 | DKPMWHFRUGMUKF-GDYCBZMLSA-N |
| β-Muricholic acid | 5283853 | DKPMWHFRUGMUKF-CRKPLTDNSA-N |
| ω-Muricholic acid | 5283851 | DKPMWHFRUGMUKF-NTPBNISXSA-N |
| Tauro-α-Muricholic acid | 137700104 | NYXROOLWUZIWRB-BAMGEBLESA-M |
| Tauro-β-Muricholic acid | 132285209 | NYXROOLWUZIWRB-GPHZYVDLSA-M |
| Tauro-ω-Muricholic acid | 146047145 | NYXROOLWUZIWRB-JTNLMKNRSA-M |
| Taurohyodeoxcholic acid |