Assay: Steroids
Targeted method quantifying up to 32 steroids using LC-MS/MS with MRM. Quantitation is performed using up to 10 calibration point curves and 5 isotopically labeled surrogate internal standards. Standard reference material (SRM) 1950 containing certified and reference values for steroids is also used.
| Steroid | InChIKey | CID |
| 11-deoxycortisol | JERGUCIJOXJXHF-TVWVXWENSA-N | 440707 |
| 11-hydroxyprogesterone | BFZHCUBIASXHPK-QJSKAATBSA-N | 92730 |
| 11-ketotestosterone | WTPMRQZHJLJSBO-XQALERBDSA-N | 5282365 |
| 17a-hydroxyprogesterone | DBPWSSGDRRHUNT-CEGNMAFCSA-N | 6238 |
| 17a-OH-pregnenolone | JERGUCIJOXJXHF-TVWVXWENSA-N | 22796600 |
| 2-methoxyestradiol | VOXZDWNPVJITMN-ZBRFXRBCSA-N | 5757 |
| 5a-dihydroprogesterone | XMRPGKVKISIQBV-BJMCWZGWSA-N | 92810 |
| 5b-dihydrocortisol | ACSFOIGNUQUIGE-AIPUTVCKSA-N | 164838 |
| 5b-dihydrocortisone | YCLWEYIBFOLMEM-FNLRALKVSA-N | 65554 |
| 5b-dihydroprogesterone | XMRPGKVKISIQBV-XWOJZHJZSA-N | 92745 |
| 5b-tetrahydrocortisol | AODPIQQILQLWGS-GXBDJPPSSA-N | 5864 |
| aldosterone | PQSUYGKTWSAVDQ-ZVIOFETBSA-N | 5839 |
| androstenedione | AEMFNILZOJDQLW-QAGGRKNESA-N | 6128 |
| b-cortol | FFPUNPBUZDTHJI-ZFOKFBPFSA-N | 246164 |
| b-cortolone | JXCOSKURGJMQSG-AIEJOZITSA-N | 245259 |
| cortexolone | JERGUCIJOXJXHF-TVWVXWENSA-N | 440707 |
| corticosterone | OMFXVFTZEKFJBZ-HJTSIMOOSA-N | 5753 |
| cortisol | JYGXADMDTFJGBT-LTVXCZLVSA-N | 5754 |
| cortisol sulfate | DJKQHUMDQFOLHE-WDCKKOMHSA-M | 102172 |
| cortisone | MFYSYFVPBJMHGN-ZPOLXVRWSA-N | 222786 |
| dehydroepiandrosterone | FMGSKLZLMKYGDP-USOAJAOKSA-N | 5881 |
| dehydroepiandrosterone sulfate | GFJWACFSUSFUOG-ZJTJBYBXSA-M | 12594 |
| deoxycorticosterone | VPGRYOFKCNULNK-ACXQXYJUSA-N | 5952 |
| etiocholanolone | QGXBDMJGAMFCBF-BNSUEQOYSA-N | 5880 |
| pregnenolone | ORNBQBCIOKFOEO-QGVNFLHTSA-N | 8955 |
| progesterone | RJKFOVLPORLFTN-LEKSSAKUSA-N | 5994 |
| testosterone | MUMGGOZAMZWBJJ-DYKIIFRCSA-N | 6013 |
| tetrahydrocortisone | SYGWGHVTLUBCEM-ZIZPXRJBSA-N | 5866 |
| trans-androsterone | QGXBDMJGAMFCBF-LUJOEAJASA-N | 441302 |
The following estrogens listed below are detected when present at relatively high levels. Depending on your study, we may need to use a specific estrogen-focused LC-MS assay in order to detect them at low levels. Please contact us at metabolomics@illinois.edu to inquire further.
| Steroid | InChIKey | CID |
| estradiol | VOXZDWNPVJITMN-ZBRFXRBCSA-N | 5757 |
| estriol | PROQIPRRNZUXQM-ZXXIGWHRSA-N | 5756 |
| estrone | DNXHEGUUPJUMQT-CBZIJGRNSA-N | 5870 |