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University of Illinois

Assay: Steroids

Targeted method quantifying up to 32 steroids using LC-MS/MS with MRM. Quantitation is performed using up to 10 calibration point curves and 5 isotopically labeled surrogate internal standards. Standard reference material (SRM) 1950 containing certified and reference values for steroids is also used. 

Steroid InChIKey CID
11-deoxycortisol JERGUCIJOXJXHF-TVWVXWENSA-N 440707
11-hydroxyprogesterone BFZHCUBIASXHPK-QJSKAATBSA-N 92730
11-ketotestosterone WTPMRQZHJLJSBO-XQALERBDSA-N 5282365
17a-hydroxyprogesterone DBPWSSGDRRHUNT-CEGNMAFCSA-N 6238
17a-OH-pregnenolone JERGUCIJOXJXHF-TVWVXWENSA-N 22796600
2-methoxyestradiol VOXZDWNPVJITMN-ZBRFXRBCSA-N 5757
5a-dihydroprogesterone XMRPGKVKISIQBV-BJMCWZGWSA-N 92810
5b-dihydrocortisol ACSFOIGNUQUIGE-AIPUTVCKSA-N 164838
5b-dihydrocortisone YCLWEYIBFOLMEM-FNLRALKVSA-N 65554
5b-dihydroprogesterone XMRPGKVKISIQBV-XWOJZHJZSA-N 92745
5b-tetrahydrocortisol AODPIQQILQLWGS-GXBDJPPSSA-N 5864
aldosterone PQSUYGKTWSAVDQ-ZVIOFETBSA-N 5839
androstenedione AEMFNILZOJDQLW-QAGGRKNESA-N 6128
b-cortol FFPUNPBUZDTHJI-ZFOKFBPFSA-N 246164
b-cortolone JXCOSKURGJMQSG-AIEJOZITSA-N 245259
cortexolone JERGUCIJOXJXHF-TVWVXWENSA-N 440707
corticosterone OMFXVFTZEKFJBZ-HJTSIMOOSA-N 5753
cortisol JYGXADMDTFJGBT-LTVXCZLVSA-N 5754
cortisol sulfate DJKQHUMDQFOLHE-WDCKKOMHSA-M 102172
cortisone MFYSYFVPBJMHGN-ZPOLXVRWSA-N 222786
dehydroepiandrosterone FMGSKLZLMKYGDP-USOAJAOKSA-N 5881
dehydroepiandrosterone sulfate GFJWACFSUSFUOG-ZJTJBYBXSA-M 12594
deoxycorticosterone VPGRYOFKCNULNK-ACXQXYJUSA-N 5952
etiocholanolone  QGXBDMJGAMFCBF-BNSUEQOYSA-N 5880
pregnenolone ORNBQBCIOKFOEO-QGVNFLHTSA-N 8955
progesterone RJKFOVLPORLFTN-LEKSSAKUSA-N 5994
testosterone  MUMGGOZAMZWBJJ-DYKIIFRCSA-N 6013
tetrahydrocortisone SYGWGHVTLUBCEM-ZIZPXRJBSA-N 5866
trans-androsterone QGXBDMJGAMFCBF-LUJOEAJASA-N 441302

The following estrogens listed below are detected when present at relatively high levels. Depending on your study, we may need to use a specific estrogen-focused LC-MS assay in order to detect them at low levels. Please contact us at metabolomics@illinois.edu to inquire further.

Steroid InChIKey CID
estradiol  VOXZDWNPVJITMN-ZBRFXRBCSA-N 5757
estriol PROQIPRRNZUXQM-ZXXIGWHRSA-N 5756
estrone DNXHEGUUPJUMQT-CBZIJGRNSA-N 5870